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10.1038/s41598-017-03213-x http://scihub22266oqcxt.onion/10.1038/s41598-017-03213-x C5462744!5462744 !28592876     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28592876 &cmd=llinks): Failed to open stream: HTTP request failed! 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Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation |pdf=|usr=}}{{28592876 }} gab.com Text Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28592876 #FOAvip Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100?stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation. Twit Text FOAVip Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28592876 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28592876 #FOAvip English Wikipedia <ref>{{Cite pmid|28592876 }}</ref> Elongation factor Tu on Escherichia coli isolated from urine of kidney stone patients promotes calcium oxalate crystal growth and aggregation #MMPMID28592876 Amimanan P ; Tavichakorntrakool R ; Fong-Ngern K ; Sribenjalux P ; Lulitanond A ; Prasongwatana V ; Wongkham C ; Boonsiri P ; Umka Welbat J ; Thongboonkerd V Sci Rep 2017[Jun]; 7 (1 ): 2953 PMID28592876 show ga Escherichia coli is the most common bacterium isolated from urine and stone matrix of calcium oxalate (CaOx) stone formers. Whether it has pathogenic role(s) in kidney stone formation or is only entrapped inside the stone remains unclear. We thus evaluated differences between E. coli isolated from urine of patients with kidney stone (EUK) and that from patients with urinary tract infection (UTI) without stone (EUU). From 100?stone formers and 200 UTI patients, only four pairs of EUK/EUU isolates had identical antimicrobial susceptibility patterns. Proteomic analysis revealed nine common differentially expressed proteins. Among these, the greater level of elongation factor Tu (EF-Tu) in EUK was validated by Western blotting. Outer membrane vesicles (OMVs) derived from EUK had greater promoting activities on CaOx crystallization, crystal growth and aggregation as compared to those derived from EUU. Neutralizing the OMVs of EUK with monoclonal anti-EF-Tu antibody, not with an isotype antibody, significantly reduced all these OMVs-induced promoting effects. Moreover, immunofluorescence staining of EF-Tu on bacterial cell surface confirmed the greater expression of surface EF-Tu on EUK (vs. EUU). Our data indicate that surface EF-Tu and OMVs play significant roles in promoting activities of E. coli on CaOx crystallization, crystal growth and aggregation. |Calcium Oxalate/chemistry/*metabolism [MESH] |Crystallization [MESH] |Escherichia coli Infections/*complications/*microbiology [MESH] |Escherichia coli/genetics/*metabolism [MESH] |Humans [MESH] |Kidney Calculi/*etiology/*metabolism/pathology/ultrastructure [MESH] |Peptide Elongation Factor Tu/*metabolism [MESH]Elongation factor Tu on Escherichia coli isolated from urine of kidney(epmc).pdf DeepDyve Pubget Overpricing