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10.1038/s41540-017-0015-2 http://scihub22266oqcxt.onion/10.1038/s41540-017-0015-2 C5459797!5459797!28649442     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       NPJ+Syst+Biol+Appl 2017 ; 3 (ä): ä Nephropedia Template TP {{tp|p=28649442|t=2017. Next-generation mammalian genetics toward organism-level systems biology |pdf=|usr=}}{{28649442}} gab.com Text Next-generation mammalian genetics toward organism-level systems biology JO: NPJ Syst Biol Appl http://www.kidney.de/pget.php?&tw=1&pmid=28649442 #FOAvip Organism-level systems biology in mammals aims to identify, analyze, control, and design molecular and cellular networks executing various biological functions in mammals. In particular, system-level identification and analysis of molecular and cellular networks can be accelerated by next-generation mammalian genetics. Mammalian genetics without crossing, where all production and phenotyping studies of genome-edited animals are completed within a single generation drastically reduce the time, space, and effort of conducting the systems research. Next-generation mammalian genetics is based on recent technological advancements in genome editing and developmental engineering. The process begins with introduction of double-strand breaks into genomic DNA by using site-specific endonucleases, which results in highly efficient genome editing in mammalian zygotes or embryonic stem cells. By using nuclease-mediated genome editing in zygotes, or ~100% embryonic stem cell-derived mouse technology, whole-body knock-out and knock-in mice can be produced within a single generation. These emerging technologies allow us to produce multiple knock-out or knock-in strains in high-throughput manner. In this review, we discuss the basic concepts and related technologies as well as current challenges and future opportunities for next-generation mammalian genetics in organism-level systems biology. Twit Text FOAVip Next-generation mammalian genetics toward organism-level systems biology ????NPJ Syst Biol Appl http://www.kidney.de/pget.php?&tw=1&pmid=28649442 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28649442 #FOAvip English Wikipedia <ref>{{Cite pmid|28649442}}</ref> Next-generation mammalian genetics toward organism-level systems biology #MMPMID28649442 Susaki EA; Ukai H; Ueda HR NPJ Syst Biol Appl 2017[]; 3 (ä): ä PMID28649442show ga Organism-level systems biology in mammals aims to identify, analyze, control, and design molecular and cellular networks executing various biological functions in mammals. In particular, system-level identification and analysis of molecular and cellular networks can be accelerated by next-generation mammalian genetics. Mammalian genetics without crossing, where all production and phenotyping studies of genome-edited animals are completed within a single generation drastically reduce the time, space, and effort of conducting the systems research. Next-generation mammalian genetics is based on recent technological advancements in genome editing and developmental engineering. The process begins with introduction of double-strand breaks into genomic DNA by using site-specific endonucleases, which results in highly efficient genome editing in mammalian zygotes or embryonic stem cells. By using nuclease-mediated genome editing in zygotes, or ~100% embryonic stem cell-derived mouse technology, whole-body knock-out and knock-in mice can be produced within a single generation. These emerging technologies allow us to produce multiple knock-out or knock-in strains in high-throughput manner. In this review, we discuss the basic concepts and related technologies as well as current challenges and future opportunities for next-generation mammalian genetics in organism-level systems biology. äNext-generation mammalian genetics toward organism-level systems biolo(epmc).pdf DeepDyve Pubget Overpricing