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2017 ; 7
(1
): 116
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Comparative metabolomics in vanilla pod and vanilla bean revealing the
biosynthesis of vanillin during the curing process of vanilla
#MMPMID28587440
Gu F
; Chen Y
; Hong Y
; Fang Y
; Tan L
AMB Express
2017[Dec]; 7
(1
): 116
PMID28587440
show ga
High-performance liquid chromatography-mass spectrometry (LC-MS) was used for
comprehensive metabolomic fingerprinting of vanilla fruits prepared from the
curing process. In this study, the metabolic changes of vanilla pods and vanilla
beans were characterized using MS-based metabolomics to elucidate the
biosynthesis of vanillin. The vanilla pods were significantly different from
vanilla beans. Seven pathways of vanillin biosynthesis were constructed, namely,
glucovanillin, glucose, cresol, capsaicin, vanillyl alcohol, tyrosine, and
phenylalanine pathways. Investigations demonstrated that glucose, cresol,
capsaicin, and vanillyl alcohol pathway were detected in a wide range of
distribution in microbial metabolism. Thus, microorganisms might have
participated in vanillin biosynthesis during vanilla curing. Furthermore, the ion
strength of glucovanillin was stable, which indicated that glucovanillin only
participated in the vanillin biosynthesis during the curing of vanilla.