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2015 ; 8
(11
): 7889-7899
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A Direct Electric Field-Aided Biomimetic Mineralization System for Inducing the
Remineralization of Dentin Collagen Matrix
#MMPMID28793685
Wu XT
; Mei ML
; Li QL
; Cao CY
; Chen JL
; Xia R
; Zhang ZH
; Chu CH
Materials (Basel)
2015[Nov]; 8
(11
): 7889-7899
PMID28793685
show ga
This in vitro study aimed to accelerate the remineralization of a completely
demineralized dentine collagen block in order to regenerate the dentinal
microstructure of calcified collagen fibrils by a novel electric field-aided
biomimetic mineralization system in the absence of non-collagenous proteins.
Completely demineralized human dentine slices were prepared using ethylene
diamine tetraacetic acid (EDTA) and treated with guanidine hydrochloride to
extract the bound non-collagenous proteins. The completely demineralized dentine
collagen blocks were then remineralized in a calcium chloride agarose hydrogel
and a sodium hydrogen phosphate and fluoride agarose hydrogel. This process was
accelerated by subjecting the hydrogels to electrophoresis at 20 mA for 4 and 12
h. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive
X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) were used to
evaluate the resultant calcification of the dentin collagen matrix. SEM indicated
that mineral particles were precipitated on the intertubular dentin collagen
matrix; these densely packed crystals mimicked the structure of the original
mineralized dentin. However, the dentinal tubules were not occluded by the
mineral crystals. XRD and EDX both confirmed that the deposited crystals were
fluorinated hydroxyapatite. TEM revealed the existence of intrafibrillar and
interfibrillar mineralization of the collagen fibrils. A novel electric
field-aided biomimetic mineralization system was successfully developed to
remineralize a completely demineralized dentine collagen matrix in the absence of
non-collagenous proteins. This study developed an accelerated biomimetic
mineralization system which can be a potential protocol for the biomineralization
of dentinal defects.
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