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10.5483/BMBRep.2017.50.5.044 http://scihub22266oqcxt.onion/10.5483/BMBRep.2017.50.5.044 C5458674!5458674!28288698     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMB+Rep 2017 ; 50 (5): 247-56 Nephropedia Template TP {{tp|p=28288698|t=2017. CRISPR system for genome engineering: the application for autophagy study |pdf=|usr=}}{{28288698}} gab.com Text CRISPR system for genome engineering: the application for autophagy study JO: BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=28288698 #FOAvip CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research. Twit Text FOAVip CRISPR system for genome engineering: the application for autophagy study ????BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=28288698 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28288698 #FOAvip English Wikipedia <ref>{{Cite pmid|28288698}}</ref> CRISPR system for genome engineering: the application for autophagy study #MMPMID28288698 Cui J; Chew SJL; Shi Y; Gong Z; Shen HM BMB Rep 2017[May]; 50 (5): 247-56 PMID28288698show ga CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research. äCRISPR system for genome engineering: the application for autophagy st(epmc).pdf DeepDyve Pubget Overpricing