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10.1002/2211-5463.12222 http://scihub22266oqcxt.onion/10.1002/2211-5463.12222 C5458474!5458474!28593142     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       FEBS+Open+Bio 2017 ; 7 (6): 877-86 Nephropedia Template TP {{tp|p=28593142|t=2017. Spontaneous formation of neutrophil extracellular traps in serum?free culture conditions |pdf=|usr=}}{{28593142}} gab.com Text Spontaneous formation of neutrophil extracellular traps in serum free culture conditions JO: FEBS Open Bio http://www.kidney.de/pget.php?&tw=1&pmid=28593142 #FOAvip Neutrophils play a critical role in the innate immune response. Recently, a new neutrophilic biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. Neutrophils have been observed to release both lysosomal enzymes and their nuclear contents, including unfolded chromatin, which together trap and inactivate bacteria. The environment in tissues where neutrophils act is thought to be different from that of the blood serum. In this study, we assessed the effect of serum on NET formation. We found that neutrophils spontaneously form NETs in serum?free cultivation conditions at early times. These NETs functioned properly to trap bacteria. Furthermore, we demonstrated that reactive oxygen species play a critical role in the spontaneous formation of NETs. These results suggest that the serum condition must be considered in studies on neutrophils, including the formation and mechanism of action of NETs. Twit Text FOAVip Spontaneous formation of neutrophil extracellular traps in serum free culture conditions ????FEBS Open Bio http://www.kidney.de/pget.php?&tw=1&pmid=28593142 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28593142 #FOAvip English Wikipedia <ref>{{Cite pmid|28593142}}</ref> Spontaneous formation of neutrophil extracellular traps in serum?free culture conditions #MMPMID28593142 Kamoshida G; Kikuchi?Ueda T; Nishida S; Tansho?Nagakawa S; Kikuchi H; Ubagai T; Ono Y FEBS Open Bio 2017[Jun]; 7 (6): 877-86 PMID28593142show ga Neutrophils play a critical role in the innate immune response. Recently, a new neutrophilic biological defense mechanism, termed neutrophil extracellular traps (NETs), has been attracting attention. Neutrophils have been observed to release both lysosomal enzymes and their nuclear contents, including unfolded chromatin, which together trap and inactivate bacteria. The environment in tissues where neutrophils act is thought to be different from that of the blood serum. In this study, we assessed the effect of serum on NET formation. We found that neutrophils spontaneously form NETs in serum?free cultivation conditions at early times. These NETs functioned properly to trap bacteria. Furthermore, we demonstrated that reactive oxygen species play a critical role in the spontaneous formation of NETs. These results suggest that the serum condition must be considered in studies on neutrophils, including the formation and mechanism of action of NETs. äSpontaneous formation of neutrophil extracellular traps in serum?free (epmc).pdf DeepDyve Pubget Overpricing