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2017 ; 18
(5
): ä Nephropedia Template TP
gab.com Text
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English Wikipedia
Identifying the Genes Regulated by AtWRKY6 Using Comparative Transcript and
Proteomic Analysis under Phosphorus Deficiency
#MMPMID28498313
Li LQ
; Huang LP
; Pan G
; Liu L
; Wang XY
; Lu LM
Int J Mol Sci
2017[May]; 18
(5
): ä PMID28498313
show ga
Phosphorus (P) is an important mineral nutrient for plant growth and development.
Overexpressing AtWRKY6 (35S:WRKY6-9) was more sensitive and wrky6 (wrky6-1) was
more resistant under low Pi conditions. To better understand the function of
AtWRKY6 under low phosphate stress conditions, we applied two-dimensional gel
electrophoresis (2-DE) to analyse differentially expressed proteins in the shoots
and roots between wild type, 35S:WRKY6-9 and wrky6-1 after phosphorus deficiency
treatment for three days. The results showed 88 differentially abundant protein
spots, which were identified between the shoots and roots of 35S:WRKY6-9 and
wrky6-1 plants. In addition, 59 differentially expressed proteins were identified
in the leaves and roots of 35S:WRKY6-9 plants. After analysis, 9 genes with W-box
elements in their promoter sequences were identified in the leaves, while 6 genes
with W-box elements in their promoter sequences were identified in the roots. A
total of 8 genes were identified as potential target genes according to the
quantitative PCR (QPCR) and two dimension difference gel electrophoresis,
(2D-DIGE) results, including ATP synthase, gln synthetase, nitrilase, 14-3-3
protein, carbonic anhydrases 2, and tryptophan synthase. These results provide
important information concerning the AtWRKY6 regulation network and reveal
potential vital target genes of AtWRKY6 under low phosphorus stress. two
dimension difference gel electrophoresis, 2D-DIGE.