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10.1002/cbic.201200582 http://scihub22266oqcxt.onion/10.1002/cbic.201200582 C5453672!5453672!23307594     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Chembiochem 2013 ; 14 (2): 251-9 Nephropedia Template TP {{tp|p=23307594|t=2013. Capture of uropathogenic Escherichia coli using synthetic glycan ligands specific for the Pappilus |pdf=|usr=}}{{23307594}} gab.com Text Capture of uropathogenic Escherichia coli using synthetic glycan ligands specific for the Pappilus JO: Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=23307594 #FOAvip Biotinylated mono and bi- antennary di/trisaccharides were synthesized to evaluate their ability to capture Escherichia coli strains that express pilus types with different receptor specificities. The synthesized biotinylated di/trisaccharides contain Gal?1-4Gal, GalNHAc?1-4Gal, Gal?1-4GalNHAc, Gal?1-4Gal?1-4Glc and GalNHAc?1-4Gal?1-4Glc as carbohydrate epitopes. These biotinylated oligosaccharides were immobilized on streptavidin coated magnetic beads, incubated with different strains of live E. coli. Capturing ability was assessed using a luciferase assay which detects bacterial ATP. The trisaccharides containing Gal?1-4Gal?1-4Glc and the disaccharides containing Gal?1-4Gal as the epitopes exhibited strong capturing ability for uropathogenic E.coli strains with the pap pilus genotype, including CFT073, J96 and J96 pilE. The same ligands failed to capture E.coli strains with fim, prs, or foc genotypes. Uropathogenic CFT073 was also captured moderately by biantennary disaccharide containing GalNHAc moiety at the reducing end; however, other saccharides containing GalNHAc at the non-reducing end did not capture the CFT073 strain. These synthetic glycoconjugates could potentially be adapted as rapid diagnostic agents to differentiate between different E. coli pathovars. Twit Text FOAVip Capture of uropathogenic Escherichia coli using synthetic glycan ligands specific for the Pappilus ????Chembiochem http://www.kidney.de/pget.php?&tw=1&pmid=23307594 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23307594 #FOAvip English Wikipedia <ref>{{Cite pmid|23307594}}</ref> Capture of uropathogenic Escherichia coli using synthetic glycan ligands specific for the Pappilus #MMPMID23307594 Yosief HO; Weiss PAA; S.Iyer PS Chembiochem 2013[Jan]; 14 (2): 251-9 PMID23307594show ga Biotinylated mono and bi- antennary di/trisaccharides were synthesized to evaluate their ability to capture Escherichia coli strains that express pilus types with different receptor specificities. The synthesized biotinylated di/trisaccharides contain Gal?1-4Gal, GalNHAc?1-4Gal, Gal?1-4GalNHAc, Gal?1-4Gal?1-4Glc and GalNHAc?1-4Gal?1-4Glc as carbohydrate epitopes. These biotinylated oligosaccharides were immobilized on streptavidin coated magnetic beads, incubated with different strains of live E. coli. Capturing ability was assessed using a luciferase assay which detects bacterial ATP. The trisaccharides containing Gal?1-4Gal?1-4Glc and the disaccharides containing Gal?1-4Gal as the epitopes exhibited strong capturing ability for uropathogenic E.coli strains with the pap pilus genotype, including CFT073, J96 and J96 pilE. The same ligands failed to capture E.coli strains with fim, prs, or foc genotypes. Uropathogenic CFT073 was also captured moderately by biantennary disaccharide containing GalNHAc moiety at the reducing end; however, other saccharides containing GalNHAc at the non-reducing end did not capture the CFT073 strain. These synthetic glycoconjugates could potentially be adapted as rapid diagnostic agents to differentiate between different E. coli pathovars. äCapture of uropathogenic Escherichia coli using synthetic glycan ligan(epmc).pdf DeepDyve Pubget Overpricing