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10.21769/BioProtoc.2236 http://scihub22266oqcxt.onion/10.21769/BioProtoc.2236 C5450929!5450929!28580376     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Bio+Protoc 2017 ; 7 (8): ä Nephropedia Template TP {{tp|p=28580376|t=2017. Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging |pdf=|usr=}}{{28580376}} gab.com Text Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging JO: Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28580376 #FOAvip Fluorescence live-cell imaging by single molecule localization microscopy (SMLM) or fluorescence lifetime imaging microscopy (FLIM) in principle allows for the spatio-temporal observation of molecular patterns in individual, living cells. However, the dynamics of molecules within cells hamper their precise observation. We present here a detailed protocol for consecutive cycles of reversible cryo-arrest of living cells on a microscope that allows for a precise determination of the evolution of molecular patterns within individual living cells. The usefulness of this approach has been demonstrated by observing ligand-induced clustering of receptor tyrosine kinases as well as their activity patterns by SMLM and FLIM ( Masip et al., 2016 ). Twit Text FOAVip Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging ????Bio Protoc http://www.kidney.de/pget.php?&tw=1&pmid=28580376 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28580376 #FOAvip English Wikipedia <ref>{{Cite pmid|28580376}}</ref> Reversible Cryo-arrests of Living Cells to Pause Molecular Movements for High-resolution Imaging #MMPMID28580376 Huebinger J; Masip ME; Christmann J; Bastiaens PIH Bio Protoc 2017[Apr]; 7 (8): ä PMID28580376show ga Fluorescence live-cell imaging by single molecule localization microscopy (SMLM) or fluorescence lifetime imaging microscopy (FLIM) in principle allows for the spatio-temporal observation of molecular patterns in individual, living cells. However, the dynamics of molecules within cells hamper their precise observation. We present here a detailed protocol for consecutive cycles of reversible cryo-arrest of living cells on a microscope that allows for a precise determination of the evolution of molecular patterns within individual living cells. The usefulness of this approach has been demonstrated by observing ligand-induced clustering of receptor tyrosine kinases as well as their activity patterns by SMLM and FLIM ( Masip et al., 2016 ). äReversible Cryo-arrests of Living Cells to Pause Molecular Movements f(epmc).pdf DeepDyve Pubget Overpricing