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Anti-Double-Stranded DNA IgG Participates in Renal Fibrosis through Suppressing
the Suppressor of Cytokine Signaling 1 Signals
#MMPMID28620377
Wang P
; Yang J
; Tong F
; Duan Z
; Liu X
; Xia L
; Li K
; Xia Y
Front Immunol
2017[]; 8
(?): 610
PMID28620377
show ga
Suppressor of cytokine signaling 1 (SOCS1) participates in renal fibrosis by
downregulating Janus kinase 2 (JAK2)/signal transducer and activator of
transcription 1 (STAT1)-mediated cytokine signaling. Recently, it was found that
anti-double-stranded DNA (dsDNA) IgG induces the synthesis of profibrotic
cytokines by renal cells. To explore the potential effect of anti-dsDNA IgG on
SOCS1-mediated renal fibrosis, kidney tissues were collected from patients with
lupus nephritis (LN) as well as MRL/lpr lupus-prone mice. The SOCS1 expression
was evaluated in tissue samples. In addition, SCID mice were injected with
anti-dsDNA IgG, followed by evaluation of SOCS1 levels. Renal resident cells were
cultured in vitro, receiving the stimulation of anti-dsDNA IgG and then the
measurement of SOCS1, JAK2, STAT1?, and profibrotic cytokines. Moreover, the
binding of anti-dsDNA IgG to SOCS1 kinase inhibitory region (KIR) peptide was
analyzed by surface plasmon resonance. We found that SOCS1 expression was
inhibited, but JAK2/STAT1 activation was prominent in the kidney tissues of
patients with LN, MRL/lpr mice, or anti-dsDNA IgG-injected SCID mice. The
cultured renal cells also showed SOCS1 downregulation, JAK2/STAT1 activation, and
profibrotic cytokine promotion upon anti-dsDNA IgG stimulation. Surprisingly,
anti-dsDNA IgG showed high affinity to KIR peptide and competed with JAK2 loop
for KIR. Additionally, a DNA-mimicking peptide (ALW) blocked the binding of
anti-dsDNA IgG to KIR, and even partially abrogated the activation of JAK2/STAT1?
signals and the expression of profibrotic cytokines in SCID mice. In conclusion,
anti-dsDNA IgG downregulates SOCS1 expression, activates JAK2/STAT1 signals, and
contributes to renal fibrosis; its peptide blockade may restore the SOCS1
inhibitory effect on the production of profibrotic cytokine, and finally
ameliorate renal fibrosis in LN.
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