Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/nmeth.4225 http://scihub22266oqcxt.onion/10.1038/nmeth.4225 C5449203!5449203!28319113     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2017 ; 14 (6): 573-6 Nephropedia Template TP {{tp|p=28319113|t=2017. Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions |pdf=|usr=}}{{28319113}} gab.com Text Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28319113 #FOAvip We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual-guide RNAs in three cell lines, altogether comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified and these patterns replicated with combinatorial drugs at 75% precision. Based on these results we anticipate cellular context will be critical to synthetic-lethal therapies. Twit Text FOAVip Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28319113 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28319113 #FOAvip English Wikipedia <ref>{{Cite pmid|28319113}}</ref> Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions #MMPMID28319113 Shen JP; Zhao D; Sasik R; Luebeck J; Birmingham A; Bojorquez-Gomez A; Licon K; Klepper K; Pekin D; Beckett A; Sanchez K; Thomas A; Kuo CC; Du D; Roguev A; Lewis NE; Chang AN; Kreisberg JF; Krogan N; Qi L; Ideker T; Mali P Nat Methods 2017[Jun]; 14 (6): 573-6 PMID28319113show ga We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual-guide RNAs in three cell lines, altogether comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were identified and these patterns replicated with combinatorial drugs at 75% precision. Based on these results we anticipate cellular context will be critical to synthetic-lethal therapies. äCombinatorial CRISPR-Cas9 screens for de novo mapping of genetic inter(epmc).pdf DeepDyve Pubget Overpricing