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10.1080/15476286.2016.1196320

http://scihub22266oqcxt.onion/10.1080/15476286.2016.1196320
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C5449080!5449080!27315491
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suck abstract from ncbi


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pmid27315491      RNA+Biol 2017 ; 14 (5): 488-99
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  • Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing #MMPMID27315491
  • Liu XS; Fan BY; Pan WL; Li C; Levin AM; Wang X; Zhang RL; Zervos TM; Hu J; Zhang XM; Chopp M; Zhang ZG
  • RNA Biol 2017[]; 14 (5): 488-99 PMID27315491show ga
  • Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.
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