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10.1080/15476286.2016.1196320 http://scihub22266oqcxt.onion/10.1080/15476286.2016.1196320 C5449080!5449080 !27315491     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27315491 &cmd=llinks): Failed to open stream: HTTP request failed! 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Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing |pdf=|usr=}}{{27315491 }} gab.com Text Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=27315491 #FOAvip Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions. Twit Text FOAVip Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=27315491 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27315491 #FOAvip English Wikipedia <ref>{{Cite pmid|27315491 }}</ref> Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing #MMPMID27315491 Liu XS ; Fan BY ; Pan WL ; Li C ; Levin AM ; Wang X ; Zhang RL ; Zervos TM ; Hu J ; Zhang XM ; Chopp M ; Zhang ZG RNA Biol 2017[May]; 14 (5 ): 488-499 PMID27315491 show ga Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions. |Adult [MESH] |Analysis of Variance [MESH] |Animals [MESH] |Argonaute Proteins/genetics/*metabolism [MESH] |Cell Proliferation [MESH] |Humans [MESH] |Lateral Ventricles/chemistry [MESH] |Male [MESH] |MicroRNAs/analysis/genetics/*metabolism [MESH] |Neural Stem Cells/*metabolism/pathology [MESH] |Neurogenesis/*physiology [MESH] |Primary Cell Culture [MESH] |Rats [MESH] |Rats, Wistar [MESH] |Sequence Analysis, RNA [MESH] |Stroke/*metabolism/pathology [MESH]Identification of miRNomes associated with adult neurogenesis after st(epmc).pdf DeepDyve Pubget Overpricing