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10.3389/fmicb.2017.00953 http://scihub22266oqcxt.onion/10.3389/fmicb.2017.00953 C5447691!5447691!28611752     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Microbiol 2017 ; 8 (ä): ä Nephropedia Template TP {{tp|p=28611752|t=2017. Ecthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa?|pdf=|usr=}}{{28611752}} gab.com Text Ecthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa JO: Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28611752 #FOAvip Background: Ecthyma gangrenosum (EG) are necrotic lesions that develop in the context of Pseudomonas aeruginosa bacteremia. Isolated reports describe EG in the setting of non-Pseudomonal infections. In a patient with EG, initial blood cultures showed Escherichia coli, and almost occulted P. aeruginosa bacteremia. Based on the clinical picture we suspected preponderant P. aeruginosa bacteremia, outgrown by concomitant low-grade E. coli bacteremia in the blood culture vials.Methods: We performed quantitative polymerase chain reaction (PCR) assays with specific primers for P. aeruginosa and E. coli on blood collected at the same time for blood cultures. We also performed quantitative cultures of the strains isolated from the patient?s blood.Results: Quantitative PCR showed that there were 1.5 × 10E7 copies/milliliter (ml) of P. aeruginosa DNA, whereas the quantity of E. coli DNA was below the detection limit of 2 × 10E4 copies/ml. We estimated that there was at least 1000 times more P. aeruginosa than E. coli. Quantitative cultures showed that E. coli grew faster than P. aeruginosa.Conclusion: Our patient with EG had preponderant P. aeruginosa bacteremia, that was almost occulted by concomitant low-grade E. coli bacteremia. Quantitative PCR was complementary to blood cultures in the final microbiological diagnosis, and proved beneficial in establishing the etiology of EG. This may question the existence of non-Pseudomonal EG, and also shows that blood culture results do not always reflect an ?exact picture? of what happens in the patient?s blood at the time of sampling. This case illustrates the importance of communication between the clinician and the microbiology laboratory to ensure best possible results. Twit Text FOAVip Ecthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa ????Front Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=28611752 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28611752 #FOAvip English Wikipedia <ref>{{Cite pmid|28611752}}</ref> Ecthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa? #MMPMID28611752 Abbas M; Emonet S; Köhler T; Renzi G; van Delden C; Schrenzel J; Hirschel B Front Microbiol 2017[]; 8 (ä): ä PMID28611752show ga Background: Ecthyma gangrenosum (EG) are necrotic lesions that develop in the context of Pseudomonas aeruginosa bacteremia. Isolated reports describe EG in the setting of non-Pseudomonal infections. In a patient with EG, initial blood cultures showed Escherichia coli, and almost occulted P. aeruginosa bacteremia. Based on the clinical picture we suspected preponderant P. aeruginosa bacteremia, outgrown by concomitant low-grade E. coli bacteremia in the blood culture vials.Methods: We performed quantitative polymerase chain reaction (PCR) assays with specific primers for P. aeruginosa and E. coli on blood collected at the same time for blood cultures. We also performed quantitative cultures of the strains isolated from the patient?s blood.Results: Quantitative PCR showed that there were 1.5 × 10E7 copies/milliliter (ml) of P. aeruginosa DNA, whereas the quantity of E. coli DNA was below the detection limit of 2 × 10E4 copies/ml. We estimated that there was at least 1000 times more P. aeruginosa than E. coli. Quantitative cultures showed that E. coli grew faster than P. aeruginosa.Conclusion: Our patient with EG had preponderant P. aeruginosa bacteremia, that was almost occulted by concomitant low-grade E. coli bacteremia. Quantitative PCR was complementary to blood cultures in the final microbiological diagnosis, and proved beneficial in establishing the etiology of EG. This may question the existence of non-Pseudomonal EG, and also shows that blood culture results do not always reflect an ?exact picture? of what happens in the patient?s blood at the time of sampling. This case illustrates the importance of communication between the clinician and the microbiology laboratory to ensure best possible results. äEcthyma Gangrenosum: Escherichia coli or Pseudomonas aeruginosa?(epmc).pdf DeepDyve Pubget Overpricing