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10.1093/bioinformatics/btx054

http://scihub22266oqcxt.onion/10.1093/bioinformatics/btx054
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C5447237!5447237!28158442
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suck abstract from ncbi


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pmid28158442      Bioinformatics 2017 ; 33 (11): 1689-95
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  • Automated cell type discovery and classification through knowledge transfer #MMPMID28158442
  • Lee HC; Kosoy R; Becker CE; Dudley JT; Kidd BA
  • Bioinformatics 2017[Jun]; 33 (11): 1689-95 PMID28158442show ga
  • Motivation: Recent advances in mass cytometry allow simultaneous measurements of up to 50 markers at single-cell resolution. However, the high dimensionality of mass cytometry data introduces computational challenges for automated data analysis and hinders translation of new biological understanding into clinical applications. Previous studies have applied machine learning to facilitate processing of mass cytometry data. However, manual inspection is still inevitable and becoming the barrier to reliable large-scale analysis. Results: We present a new algorithm called Automated Cell-type Discovery and Classification (ACDC) that fully automates the classification of canonical cell populations and highlights novel cell types in mass cytometry data. Evaluations on real-world data show ACDC provides accurate and reliable estimations compared to manual gating results. Additionally, ACDC automatically classifies previously ambiguous cell types to facilitate discovery. Our findings suggest that ACDC substantially improves both reliability and interpretability of results obtained from high-dimensional mass cytometry profiling data. Availability and Implementation: A Python package (Python 3) and analysis scripts for reproducing the results are availability on https://bitbucket.org/dudleylab/acdc. Supplementary information: Supplementary data are available at Bioinformatics online.
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