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suck abstract from ncbi


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pmid26979602      Brief+Bioinform 2017 ; 18 (2): 279-90
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  • Recent advances in ChIP-seq analysis: from quality management to whole-genome annotation #MMPMID26979602
  • Nakato R; Shirahige K
  • Brief Bioinform 2017[Mar]; 18 (2): 279-90 PMID26979602show ga
  • Chromatin immunoprecipitation followed by sequencing (ChIP-seq) analysis can detect protein/DNA-binding and histone-modification sites across an entire genome. Recent advances in sequencing technologies and analyses enable us to compare hundreds of samples simultaneously; such large-scale analysis has potential to reveal the high-dimensional interrelationship level for regulatory elements and annotate novel functional genomic regions de novo. Because many experimental considerations are relevant to the choice of a method in a ChIP-seq analysis, the overall design and quality management of the experiment are of critical importance. This review offers guiding principles of computation and sample preparation for ChIP-seq analyses, highlighting the validity and limitations of the state-of-the-art procedures at each step. We also discuss the latest challenges of single-cell analysis that will encourage a new era in this field.
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