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10.5966/sctm.2015-0226 http://scihub22266oqcxt.onion/10.5966/sctm.2015-0226 C5442803!5442803 !28191765     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28191765 &cmd=llinks): Failed to open stream: HTTP request failed! 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Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells |pdf=|usr=}}{{28191765 }} gab.com Text Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells JO: Stem Cells Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=28191765 #FOAvip Osteosarcoma (OS) was a malignant tumor occurring with unknown etiology that made prevention and early diagnosis difficult. Mesenchymal stem cells (MSCs), which were found in bone marrow, were claimed to be a possible origin of OS but with little direct evidence. We aimed to characterize OS cells transformed from human MSCs (hMSCs) and identify their association with human primary OS cells and patient survival. Genetic modification with p53 or retinoblastoma (Rb) knockdown and c-Myc or Ras overexpression was applied for hMSC transformation. Transformed cells were assayed for proliferation, differentiation, tumorigenecity, and gene expression profile. Only the combination of Rb knockdown and c-Myc overexpression successfully transformed hMSCs derived from four individual donors, with increasing cell proliferation, decreasing cell senescence rate, and increasing ability to form colonies and spheres in serum-free medium. These transformed cells lost the expression of certain surface markers, increased in osteogenic potential, and decreased in adipogenic potential. After injection in immunodeficient mice, these cells formed OS-like tumors, as evidenced by radiographic analyses and immunohistochemistry of various OS markers. Microarray with cluster analysis revealed that these transformed cells have gene profiles more similar to patient-derived primary OS cells than their normal MSC counterparts. Most importantly, comparison of OS patient tumor samples revealed that a combination of Rb loss and c-Myc overexpression correlated with a decrease in patient survival. This study successfully transformed human MSCs to OS-like cells by Rb knockdown and c-Myc overexpression that may be a useful platform for further investigation of preventive and target therapy for human OS. Stem Cells Translational Medicine 2017;6:512-526. Twit Text FOAVip Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells ????Stem Cells Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=28191765 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28191765 #FOAvip English Wikipedia <ref>{{Cite pmid|28191765 }}</ref> Generation of Osteosarcomas from a Combination of Rb Silencing and c-Myc Overexpression in Human Mesenchymal Stem Cells #MMPMID28191765 Wang JY ; Wu PK ; Chen PC ; Lee CW ; Chen WM ; Hung SC Stem Cells Transl Med 2017[Feb]; 6 (2 ): 512-526 PMID28191765 show ga Osteosarcoma (OS) was a malignant tumor occurring with unknown etiology that made prevention and early diagnosis difficult. Mesenchymal stem cells (MSCs), which were found in bone marrow, were claimed to be a possible origin of OS but with little direct evidence. We aimed to characterize OS cells transformed from human MSCs (hMSCs) and identify their association with human primary OS cells and patient survival. Genetic modification with p53 or retinoblastoma (Rb) knockdown and c-Myc or Ras overexpression was applied for hMSC transformation. Transformed cells were assayed for proliferation, differentiation, tumorigenecity, and gene expression profile. Only the combination of Rb knockdown and c-Myc overexpression successfully transformed hMSCs derived from four individual donors, with increasing cell proliferation, decreasing cell senescence rate, and increasing ability to form colonies and spheres in serum-free medium. These transformed cells lost the expression of certain surface markers, increased in osteogenic potential, and decreased in adipogenic potential. After injection in immunodeficient mice, these cells formed OS-like tumors, as evidenced by radiographic analyses and immunohistochemistry of various OS markers. Microarray with cluster analysis revealed that these transformed cells have gene profiles more similar to patient-derived primary OS cells than their normal MSC counterparts. Most importantly, comparison of OS patient tumor samples revealed that a combination of Rb loss and c-Myc overexpression correlated with a decrease in patient survival. This study successfully transformed human MSCs to OS-like cells by Rb knockdown and c-Myc overexpression that may be a useful platform for further investigation of preventive and target therapy for human OS. Stem Cells Translational Medicine 2017;6:512-526. |*Gene Silencing [MESH] |Adipogenesis [MESH] |Animals [MESH] |Bone Neoplasms/genetics/*metabolism/pathology [MESH] |Cell Proliferation [MESH] |Cell Transformation, Neoplastic/genetics/*metabolism/pathology [MESH] |Cellular Senescence [MESH] |Gene Expression Regulation, Neoplastic [MESH] |Humans [MESH] |Mesenchymal Stem Cell Transplantation [MESH] |Mesenchymal Stem Cells/*metabolism/pathology [MESH] |Mice, Nude [MESH] |Osteogenesis [MESH] |Osteosarcoma/genetics/*metabolism/secondary [MESH] |Phenotype [MESH] |Primary Cell Culture [MESH] |Proto-Oncogene Proteins c-myc/*genetics/*metabolism [MESH] |Retinoblastoma Protein/*deficiency/genetics [MESH] |Time Factors [MESH] |Transcriptome [MESH] |Tumor Burden [MESH] |Tumor Cells, Cultured [MESH]Generation of Osteosarcomas from a Combination of Rb Silencing and c-M(epmc).pdf DeepDyve Pubget Overpricing