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10.1038/s41598-017-01766-5 http://scihub22266oqcxt.onion/10.1038/s41598-017-01766-5 C5440395!5440395!28533524     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2017 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28533524|t=2017. Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries |pdf=|usr=}}{{28533524}} gab.com Text Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28533524 #FOAvip CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human and 40,860 sgRNAs for the mouse genome. For flexibility in generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluorescent and drug selection markers. Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays. Furthermore, sgRNA targeting GPI anchor protein pathway genes induced loss of function mutations in human and mouse cell lines measured by FLAER labelling. These arrayed libraries offer the prospect for performing screens on individual genes, combinations as well as larger gene sets. They also facilitate rapid deconvolution of signals from genome-wide screens. This set of vectors provide an organized comprehensive gene editing toolbox of considerable scientific value. Twit Text FOAVip Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=28533524 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28533524 #FOAvip English Wikipedia <ref>{{Cite pmid|28533524}}</ref> Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries #MMPMID28533524 Metzakopian E; Strong A; Iyer V; Hodgkins A; Tzelepis K; Antunes L; Friedrich MJ; Kang Q; Davidson T; Lamberth J; Hoffmann C; Davis GD; Vassiliou GS; Skarnes WC; Bradley A Sci Rep 2017[]; 7 (ä): ä PMID28533524show ga CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries covering 17,166 human and 20,430 mouse genes at a complexity of 34,332 sgRNAs for human and 40,860 sgRNAs for the mouse genome. For flexibility in generating stable cell lines the sgRNAs have been cloned in a lentivirus backbone containing PiggyBac transposase recognition elements together with fluorescent and drug selection markers. Over 95% of tested sgRNA induced specific DNA cleavage as measured by CEL-1 assays. Furthermore, sgRNA targeting GPI anchor protein pathway genes induced loss of function mutations in human and mouse cell lines measured by FLAER labelling. These arrayed libraries offer the prospect for performing screens on individual genes, combinations as well as larger gene sets. They also facilitate rapid deconvolution of signals from genome-wide screens. This set of vectors provide an organized comprehensive gene editing toolbox of considerable scientific value. äEnhancing the genome editing toolbox: genome wide CRISPR arrayed libra(epmc).pdf DeepDyve Pubget Overpricing