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10.1126/science.aag2445 http://scihub22266oqcxt.onion/10.1126/science.aag2445 C5438575!5438575!27708057     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27708057&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2016 ; 354 (6313): 769-73 Nephropedia Template TP {{tp|p=27708057|t=2016. Systematic mapping of functional enhancer-promoter connections with CRISPR interference |pdf=|usr=}}{{27708057}} gab.com Text Systematic mapping of functional enhancer-promoter connections with CRISPR interference JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=27708057 #FOAvip Gene expression in mammals is regulated by noncoding elements that can impact physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. We assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. Quantitative features of chromatin state and chromosome conformation distinguish the 7 enhancers that regulate MYC from other elements that do not, suggesting a strategy for predicting enhancer-promoter connectivity. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease. Twit Text FOAVip Systematic mapping of functional enhancer-promoter connections with CRISPR interference ????Science http://www.kidney.de/pget.php?&tw=1&pmid=27708057 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27708057 #FOAvip English Wikipedia <ref>{{Cite pmid|27708057}}</ref> Systematic mapping of functional enhancer-promoter connections with CRISPR interference #MMPMID27708057 Fulco CP; Munschauer M; Anyoha R; Munson G; Grossman SR; Perez EM; Kane M; Cleary B; Lander ES; Engreitz JM Science 2016[Nov]; 354 (6313): 769-73 PMID27708057show ga Gene expression in mammals is regulated by noncoding elements that can impact physiology and disease, yet the functions and target genes of most noncoding elements remain unknown. We present a high-throughput approach that uses CRISPR interference (CRISPRi) to discover regulatory elements and identify their target genes. We assess >1 megabase (Mb) of sequence in the vicinity of 2 essential transcription factors, MYC and GATA1, and identify 9 distal enhancers that control gene expression and cellular proliferation. Quantitative features of chromatin state and chromosome conformation distinguish the 7 enhancers that regulate MYC from other elements that do not, suggesting a strategy for predicting enhancer-promoter connectivity. This CRISPRi-based approach can be applied to dissect transcriptional networks and interpret the contributions of noncoding genetic variation to human disease. äSystematic mapping of functional enhancer-promoter connections with CR(epmc).pdf DeepDyve Pubget Overpricing