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10.5483/BMBRep.2017.50.4.029 http://scihub22266oqcxt.onion/10.5483/BMBRep.2017.50.4.029 C5437957!5437957!28228216     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMB+Rep 2017 ; 50 (4): 158-9 Nephropedia Template TP {{tp|p=28228216|t=2017. Non-canonical targets play an important role in microRNA stability control mechanisms |pdf=|usr=}}{{28228216}} gab.com Text Non-canonical targets play an important role in microRNA stability control mechanisms JO: BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=28228216 #FOAvip MicroRNAs (miRNAs) regulate gene expression by guiding the Argonaute (Ago)-containing RNA-induced silencing complex (RISC) to specific target mRNA molecules. It is well established that miRNAs are stabilized by Ago proteins, but the molecular features that trigger miRNA destabilization from Ago proteins remain largely unknown. To explore the molecular mechanisms of how targets affect the stability of miRNAs in human Ago (hAgo) proteins, we employed an in vitro system that consisted of a minimal hAgo2-RISC in HEK293T cell lysates. Surprisingly, we found that miRNAs are drastically destabilized by binding to seedless, non-canonical targets. We showed that miRNAs are destabilized at their 3? ends during this process, which is largely attributed to the conformational flexibility of the L1-PAZ domain. Based on these results, we propose that non-canonical targets may play an important regulatory role in controlling the stability of miRNAs, instead of being regulated by miRNAs. Twit Text FOAVip Non-canonical targets play an important role in microRNA stability control mechanisms ????BMB Rep http://www.kidney.de/pget.php?&tw=1&pmid=28228216 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28228216 #FOAvip English Wikipedia <ref>{{Cite pmid|28228216}}</ref> Non-canonical targets play an important role in microRNA stability control mechanisms #MMPMID28228216 Park JH; Shin C BMB Rep 2017[Apr]; 50 (4): 158-9 PMID28228216show ga MicroRNAs (miRNAs) regulate gene expression by guiding the Argonaute (Ago)-containing RNA-induced silencing complex (RISC) to specific target mRNA molecules. It is well established that miRNAs are stabilized by Ago proteins, but the molecular features that trigger miRNA destabilization from Ago proteins remain largely unknown. To explore the molecular mechanisms of how targets affect the stability of miRNAs in human Ago (hAgo) proteins, we employed an in vitro system that consisted of a minimal hAgo2-RISC in HEK293T cell lysates. Surprisingly, we found that miRNAs are drastically destabilized by binding to seedless, non-canonical targets. We showed that miRNAs are destabilized at their 3? ends during this process, which is largely attributed to the conformational flexibility of the L1-PAZ domain. Based on these results, we propose that non-canonical targets may play an important regulatory role in controlling the stability of miRNAs, instead of being regulated by miRNAs. äNon-canonical targets play an important role in microRNA stability con(epmc).pdf DeepDyve Pubget Overpricing