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10.1074/jbc.M116.762963 http://scihub22266oqcxt.onion/10.1074/jbc.M116.762963 C5437257!5437257 !28320861     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28320861 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Biol+Chem 2017 ; 292 (20 ): 8533-8543 Nephropedia Template TP {{tp|p=28320861 |t=2017. Recycling of iron via autophagy is critical for the transition from glycolytic to respiratory growth |pdf=|usr=}}{{28320861 }} gab.com Text Recycling of iron via autophagy is critical for the transition from glycolytic to respiratory growth JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28320861 #FOAvip Autophagy is a bulk degradation process conserved from yeast to mammals. To examine the roles of autophagy in cellular metabolism, we generated autophagy-defective (atg) mutants in the X2180-1B strain background. We compared the growth of wild-type (WT) and atg cells in minimal (synthetic dextrose, SD) and rich (yeast extract/peptone/dextrose, YEPD) medium, and we found that mutations in the core autophagy machinery result in defects in the diauxic shift, the transition from fermentation to respiratory growth upon glucose depletion, specifically in SD. Furthermore, we confirmed that autophagy was induced prior to the diauxic shift, implying that it plays a role in this process. In YEPD, atg mutants grew normally, so we assumed that the insufficiency of certain nutrients in SD was responsible for the defects. We ultimately identified iron, which is a necessary cofactor for respiratory activity, as the nutrient required for the diauxic shift in atg mutants. Indeed, atg mutants exhibited defects in respiration, which was rescued by supplementation with iron. Based on these data, we hypothesized that autophagy is involved in iron recycling during the diauxic shift. smf3?fet5? or smf3?ftr1? cells, which are unable to export iron from the vacuole, also exhibit defects in the diauxic shift, so iron released from the vacuole is important for the shift in SD medium. Finally, we observed that smf3?fet5? cells accumulated nearly twice as much vacuolar iron as smf3?fet5?atg2? cells, suggesting that autophagy is involved in iron recycling by the vacuolar transport and degradation of iron-containing cargos. Twit Text FOAVip Recycling of iron via autophagy is critical for the transition from glycolytic to respiratory growth ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28320861 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28320861 #FOAvip English Wikipedia <ref>{{Cite pmid|28320861 }}</ref> Recycling of iron via autophagy is critical for the transition from glycolytic to respiratory growth #MMPMID28320861 Horie T ; Kawamata T ; Matsunami M ; Ohsumi Y J Biol Chem 2017[May]; 292 (20 ): 8533-8543 PMID28320861 show ga Autophagy is a bulk degradation process conserved from yeast to mammals. To examine the roles of autophagy in cellular metabolism, we generated autophagy-defective (atg) mutants in the X2180-1B strain background. We compared the growth of wild-type (WT) and atg cells in minimal (synthetic dextrose, SD) and rich (yeast extract/peptone/dextrose, YEPD) medium, and we found that mutations in the core autophagy machinery result in defects in the diauxic shift, the transition from fermentation to respiratory growth upon glucose depletion, specifically in SD. Furthermore, we confirmed that autophagy was induced prior to the diauxic shift, implying that it plays a role in this process. In YEPD, atg mutants grew normally, so we assumed that the insufficiency of certain nutrients in SD was responsible for the defects. We ultimately identified iron, which is a necessary cofactor for respiratory activity, as the nutrient required for the diauxic shift in atg mutants. Indeed, atg mutants exhibited defects in respiration, which was rescued by supplementation with iron. Based on these data, we hypothesized that autophagy is involved in iron recycling during the diauxic shift. smf3?fet5? or smf3?ftr1? cells, which are unable to export iron from the vacuole, also exhibit defects in the diauxic shift, so iron released from the vacuole is important for the shift in SD medium. Finally, we observed that smf3?fet5? cells accumulated nearly twice as much vacuolar iron as smf3?fet5?atg2? cells, suggesting that autophagy is involved in iron recycling by the vacuolar transport and degradation of iron-containing cargos. |Autophagy/*physiology [MESH] |Gene Deletion [MESH] |Glycolysis/*physiology [MESH] |Iron/*metabolism [MESH] |Oxygen Consumption/*physiology [MESH] |Saccharomyces cerevisiae Proteins/genetics/metabolism [MESH]Recycling of iron via autophagy is critical for the transition from gl(epmc).pdf DeepDyve Pubget Overpricing