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2017 ; 8
(ä): 892
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Electrochemical Potential Influences Phenazine Production, Electron Transfer and
Consequently Electric Current Generation by Pseudomonas aeruginosa
#MMPMID28572797
Bosire EM
; Rosenbaum MA
Front Microbiol
2017[]; 8
(ä): 892
PMID28572797
show ga
Pseudomonas aeruginosa has gained interest as a redox mediator (phenazines)
producer in bioelectrochemical systems. Several biotic and abiotic factors
influence the production of phenazines in synergy with the central virulence
factors production regulation. It is, however, not clear how the electrochemical
environment may influence the production and usage of phenazines by P.
aeruginosa. We here determined the influence of the electrochemical potential on
phenazine production and phenazine electron transfer capacity at selected applied
potentials from -0.4 to +0.4 V (vs. Ag/AgCl(sat)) using P. aeruginosa strain
PA14. Our study reveals a profound influence of the electrochemical potential on
the amount of phenazine-1-carboxylate production, whereby applied potentials that
were more positive than the formal potential of this dominating phenazine (E(°
')(PCA) = -0.24 V vs. Ag/AgCl(sat)) stimulated more PCA production (94, 84, 128,
and 140 ?g mL(-1) for -0.1, 0.1, 0.2, and 0.3 V, respectively) compared to more
reduced potentials (38, 75, and 7 ?g mL(-1) for -0.4, -0.3, and -0.24 V,
respectively). Interestingly, P. aeruginosa seems to produce an additional redox
mediator (with E(° ') ? 0.052 V) at applied potentials below 0 V, which is most
likely adsorbed to the electrode or present on the cells forming the biofilm
around electrodes. At fairly negative applied electrode potentials, both PCA and
the unknown redox compound mediate cathodic current generation. This study
provides important insights applicable in optimizing the BES conditions and
cultures for effective production and utilization of P. aeruginosa phenazines. It
further stimulates investigations into the physiological impacts of the
electrochemical environment, which might be decisive in the application of
phenazines for electron transfer with P. aeruginosa pure- or microbial mixed
cultures.