Measuring Vascular Permeability In Vivo #MMPMID27581015
Meijer EF; Baish JW; Padera TP; Fukumura D
Methods Mol Biol 2016[]; 1458 (ä): 71-85 PMID27581015show ga
Over the past decades, in vivo vascular permeability measurements have provided significant insight into vascular functions in physiological and pathophysiological conditions such as the response to pro- and anti-angiogenic signaling, abnormality of tumor vasculature and its normalization, and delivery and efficacy of therapeutic agents. Different approaches for vascular permeability measurements have been established. Here, we describe and discuss a conventional 2D imaging method to measure vascular permeability, which was originally documented by Gerlowski and Jain in 1986 (Microvasc Res 31:288?305, 1986) and further developed by Yuan et al. in the early 1990s (Microvasc Res 45:269?289, 1993; Cancer Res 54:352?3356, 1994), and our recently developed 3D imaging method, which advances the approach originally described by Brown et al. in 2001 (Nat Med 7:864?868, 2001).