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10.1177/2054358117699829 http://scihub22266oqcxt.onion/10.1177/2054358117699829 C5433663!5433663!28540060     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Can+J+Kidney+Health+Dis 2017 ; 4 (ä): ä Nephropedia Template TP {{tp|p=28540060|t=2017. Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study |pdf=|usr=}}{{28540060}} gab.com Text Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study JO: Can J Kidney Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=28540060 #FOAvip Background:: Injury to the mesothelial layer of the peritoneal membrane during peritoneal dialysis (PD) is implicated in loss of ultrafiltration capacity, but there are no validated biomarkers for mesothelial cell injury. Microparticles (MPs) are 0.1 to 1.0 µm membrane vesicles shed from the cell surface following injury and are sensitive markers of tissue damage. Formation of MPs in the peritoneal cavity during PD has not been reported to date. Methods:: We designed a single-center, proof of concept study to assess whether peritoneal solution exposure induces formation of mesothelial MPs suggestive of PD membrane injury. We examined MP levels in PD effluents by electron microscopy, nanoparticle tracking analysis (NTA), flow cytometry, procoagulant activity, and Western blot. Results:: NTA identified particles in the size range of 30 to 900 nm, with a mean of 240 (SE: 10 nm). MP levels increased in a progressive manner during a 4-hour PD dwell. Electron microscopy confirmed size and morphology of vesicles consistent with characteristics of MPs as well as the presence of mesothelin on the surface. Western blot analysis of the MP fraction also identified the presence of mesothelin after 4 hours, suggesting that MPs found in PD effluents may arise from mesothelial cells. Conclusions:: Our results suggest that MPs are formed and accumulate in the peritoneal cavity during PD, possibly as a stress response. Assessing levels of MPs in PD effluents may be useful as a biomarker for peritoneal membrane damage. Twit Text FOAVip Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study ????Can J Kidney Health Dis http://www.kidney.de/pget.php?&tw=1&pmid=28540060 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28540060 #FOAvip English Wikipedia <ref>{{Cite pmid|28540060}}</ref> Microparticle Formation in Peritoneal Dialysis: A Proof of Concept Study #MMPMID28540060 Akbari S; Abou-Arkoub R; Sun S; Hiremath S; Reunov A; McCormick BB; Ruzicka M; Burger D Can J Kidney Health Dis 2017[]; 4 (ä): ä PMID28540060show ga Background:: Injury to the mesothelial layer of the peritoneal membrane during peritoneal dialysis (PD) is implicated in loss of ultrafiltration capacity, but there are no validated biomarkers for mesothelial cell injury. Microparticles (MPs) are 0.1 to 1.0 µm membrane vesicles shed from the cell surface following injury and are sensitive markers of tissue damage. Formation of MPs in the peritoneal cavity during PD has not been reported to date. Methods:: We designed a single-center, proof of concept study to assess whether peritoneal solution exposure induces formation of mesothelial MPs suggestive of PD membrane injury. We examined MP levels in PD effluents by electron microscopy, nanoparticle tracking analysis (NTA), flow cytometry, procoagulant activity, and Western blot. Results:: NTA identified particles in the size range of 30 to 900 nm, with a mean of 240 (SE: 10 nm). MP levels increased in a progressive manner during a 4-hour PD dwell. Electron microscopy confirmed size and morphology of vesicles consistent with characteristics of MPs as well as the presence of mesothelin on the surface. Western blot analysis of the MP fraction also identified the presence of mesothelin after 4 hours, suggesting that MPs found in PD effluents may arise from mesothelial cells. Conclusions:: Our results suggest that MPs are formed and accumulate in the peritoneal cavity during PD, possibly as a stress response. Assessing levels of MPs in PD effluents may be useful as a biomarker for peritoneal membrane damage. äMicroparticle Formation in Peritoneal Dialysis: A Proof of Concept Stu(epmc).pdf DeepDyve Pubget Overpricing