Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=28496123
&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28496123
.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Sci+Rep
2017 ; 7
(1
): 1798
Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Systematic evaluation for effects of urine pH on calcium oxalate crystallization,
crystal-cell adhesion and internalization into renal tubular cells
#MMPMID28496123
Manissorn J
; Fong-Ngern K
; Peerapen P
; Thongboonkerd V
Sci Rep
2017[May]; 7
(1
): 1798
PMID28496123
show ga
Urine pH has been thought to be an important factor that can modulate kidney
stone formation. Nevertheless, there was no systematic evaluation of such pH
effect. Our present study thus addressed effects of differential urine pH
(4.0-8.0) on calcium oxalate (CaOx) crystallization, crystal-cell adhesion,
crystal internalization into renal tubular cells, and binding of apical membrane
proteins to the crystals. Microscopic examination revealed that CaOx monohydrate
(COM), the pathogenic form, was crystallized with greatest size, number and total
mass at pH 4.0 and least crystallized at pH 8.0, whereas COD was crystallized
with the vice versa order. Fourier-transform infrared (FT-IR) spectroscopy
confirmed such morphological study. Crystal-cell adhesion assay showed the
greatest degree of crystal-cell adhesion at the most acidic pH and least at the
most basic pH. Crystal internalization assay using fluorescein isothiocyanate
(FITC)-labelled crystals and flow cytometry demonstrated that crystal
internalization into renal tubular cells was maximal at the neutral pH (7.0).
Finally, there were no significant differences in binding capacity of the
crystals to apical membrane proteins at different pH. We concluded that the
acidic urine pH may promote CaOx kidney stone formation, whereas the basic urine
pH (i.e. by alkalinization) may help to prevent CaOx kidney stone disease.
free
free
free
