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10.1038/s41598-017-01606-6

http://scihub22266oqcxt.onion/10.1038/s41598-017-01606-6
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C5431525!5431525!28484239
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suck abstract from ncbi


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pmid28484239      Sci+Rep 2017 ; 7 (ä): ä
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  • A simple and cost-effective setup for super-resolution localization microscopy #MMPMID28484239
  • Ma H; Fu R; Xu J; Liu Y
  • Sci Rep 2017[]; 7 (ä): ä PMID28484239show ga
  • Single molecule localization microscopy (SMLM) has become a powerful imaging tool for biomedical research, but it is mostly available in imaging facilities and a small number of laboratories due to its high cost. Here, we evaluate the possibility of replacing high-cost components on standard SMLM with appropriate low-cost alternatives and build a simple but high-performance super-resolution SMLM setup. Through numerical simulation and biological experiments, we demonstrate that our low-cost SMLM setup can yield similar localization precision and spatial resolution compared to the standard SMLM equipped with state-of-the-art components, but at a small fraction of their cost. Our low-cost SMLM setup can potentially serve as a routine laboratory microscope with high-performance super-resolution imaging capability.
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