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2017 ; 13
(5
): 3681-3687
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New role of human ribosomal protein S3: Regulation of cell cycle via
phosphorylation by cyclin-dependent kinase 2
#MMPMID28521470
Han SH
; Chung JH
; Kim J
; Kim KS
; Han YS
Oncol Lett
2017[May]; 13
(5
): 3681-3687
PMID28521470
show ga
Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit
that associated in protein synthesis. hRpS3 has additional ribosomal functions
such as DNA repair, transcription, metastasis, and apoptosis via interaction with
numerous signaling molecules and has different modifications. Cyclin-dependent
kinases (CDKs) are heterodimeric serine/threonine protein kinases that regulate
cell cycle progression. Among its members, the Cdk1-cyclin B complex is known to
control cell progression in the G2/M phase, while Cdk2-cyclin E/A complexes
function in G1/S and S/G2 transition. In our previous study, we observed
interaction between hRpS3 and Cdk1. The present study investigated the
interaction between hRpS3 and Cdk2. Cdk2 phosphorylated hRps3 at amino acid
residues S6 and T221 during the S-phase. Furthermore, hRpS3 knockdown delayed
cell cycle progression by modulating the expression of cell cycle-related
proteins, including cyclin B1 and cyclin E1. These findings suggest that hRpS3 is
involved in Cdk2-mediated cell cycle regulation.