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10.1111/jcmm.12946 http://scihub22266oqcxt.onion/10.1111/jcmm.12946 C5431137!5431137 !28276635     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28276635 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Cell+Mol+Med 2017 ; 21 (6 ): 1073-1084 Nephropedia Template TP {{tp|p=28276635 |t=2017. 3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension |pdf=|usr=}}{{28276635 }} gab.com Text 3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension JO: J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28276635 #FOAvip Three-dimensional (3D) culture has been shown to improve pluripotent gene expression in mesenchymal stem cells (MSCs), but the underlining mechanisms were poorly understood. Here, we found that the relaxation of cytoskeleton tension of MSCs in 3D culture was critically associated with the expressional up-regulation of Nanog. Cultured in spheroids, MSCs showed decreased integrin-based cell-matrix adhesion but increased cadherin-based cell-cell interaction. Different from that in 2D culture, where MSCs exhibited branched and multiple-directed F-actin stress bundles at the cell edge and strengthened stress fibres transversing the cell body, MSCs cultured in spheroids showed compact cell body, relaxed cytoskeleton tension with very thin cortical actin filament outlining the cell, and increased expression of Nanog along with reduced levels of Suv39h1 (H3K9 methyltransferase) and H3K9me3. Notably, pharmaceutical inhibition of actin polymerization with cytochalasin D or silencing Suv39h1 expression with siRNA in 2D-cultured MSCs elevated the expression of Nanog via H3K9 demethylation. Thus, our data suggest that 3D culture increases the expression of Nanog through the relaxation of actin cytoskeleton, which mediates reduced Suv39h1 and H3K9me3 levels. Twit Text FOAVip 3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension ????J Cell Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=28276635 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28276635 #FOAvip English Wikipedia <ref>{{Cite pmid|28276635 }}</ref> 3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension #MMPMID28276635 Zhou Y ; Chen H ; Li H ; Wu Y J Cell Mol Med 2017[Jun]; 21 (6 ): 1073-1084 PMID28276635 show ga Three-dimensional (3D) culture has been shown to improve pluripotent gene expression in mesenchymal stem cells (MSCs), but the underlining mechanisms were poorly understood. Here, we found that the relaxation of cytoskeleton tension of MSCs in 3D culture was critically associated with the expressional up-regulation of Nanog. Cultured in spheroids, MSCs showed decreased integrin-based cell-matrix adhesion but increased cadherin-based cell-cell interaction. Different from that in 2D culture, where MSCs exhibited branched and multiple-directed F-actin stress bundles at the cell edge and strengthened stress fibres transversing the cell body, MSCs cultured in spheroids showed compact cell body, relaxed cytoskeleton tension with very thin cortical actin filament outlining the cell, and increased expression of Nanog along with reduced levels of Suv39h1 (H3K9 methyltransferase) and H3K9me3. Notably, pharmaceutical inhibition of actin polymerization with cytochalasin D or silencing Suv39h1 expression with siRNA in 2D-cultured MSCs elevated the expression of Nanog via H3K9 demethylation. Thus, our data suggest that 3D culture increases the expression of Nanog through the relaxation of actin cytoskeleton, which mediates reduced Suv39h1 and H3K9me3 levels. |*Cell Culture Techniques [MESH] |*Mesenchymal Stem Cells [MESH] |Actin Cytoskeleton/genetics [MESH] |Cell Communication/genetics [MESH] |Cell Differentiation/*genetics [MESH] |Cell Proliferation/genetics [MESH] |Cell-Matrix Junctions/genetics/metabolism [MESH] |Gene Expression Regulation, Developmental/genetics [MESH] |Histone-Lysine N-Methyltransferase/genetics [MESH] |Humans [MESH] |Methyltransferases/genetics [MESH] |Nanog Homeobox Protein/*genetics [MESH]3D culture increases pluripotent gene expression in mesenchymal stem c(epmc).pdf DeepDyve Pubget Overpricing