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10.1002/cam4.1053 http://scihub22266oqcxt.onion/10.1002/cam4.1053 C5430094!5430094!28401704     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cancer+Med 2017 ; 6 (5): 1072-81 Nephropedia Template TP {{tp|p=28401704|t=2017. HIF?1? binding to AEG?1 promoter induced upregulated AEG?1 expression associated with metastasis in ovarian cancer |pdf=|usr=}}{{28401704}} gab.com Text HIF 1 binding to AEG 1 promoter induced upregulated AEG 1 expression associated with metastasis in ovarian cancer JO: Cancer Med http://www.kidney.de/pget.php?&tw=1&pmid=28401704 #FOAvip Ovarian cancer with the highest mortality rate among gynecological malignancies is one of common cancers among female cancer patients. As reported in recent years, AEG?1 was associated with the occurrence, development, and metastasis of ovarian cancer, but the mechanisms remain unclear. In the current study, invasion capabilities of ovarian cancer OVCAR3 cells were measured by viral infection and transwell assay. Western blot analysis was used to evaluate the expression levels of ??catenin, E?cadherin, MMP2, and MMP9. With qRT?PCR analysis, AEG?1 and HIF?1? gene expression were detected. We used luciferase reporter gene to measure AEG?1 promoter activity under normoxia/hypoxia in OVCAR3 cells. Our work demonstrated that AEG?1 significantly enhanced invasion capabilities of OVCAR3 cells and the expression levels of ??catenin, E?cadherin, MMP2, and MMP9 associated with invasion capabilities of OVCAR3 cells were upregulated. Furthermore, hypoxia enhanced invasion capabilities of OVCAR3 cells and induced AEG?1 high gene expression, which was reversed by AEG?1 knockdown lentivirus. HIF?1? expression upregulation was induced in OVCAR3 cells after hypoxia. HIF?1? knockdown lentivirus induced downregulated expression of AEG?1 and invasion capabilities of OVCAR3 cells were also inhibited. Wild?type AEG?1 promoter activity under hypoxic conditions was significantly higher than that AEG?1 mutation under normoxic conditions in the absence of hypoxia response. Our results suggested that HIF?1? binds to AEG?1 promoter to upregulate its expression, which was correlated with metastasis in ovarian cancer by inducing the expression of MMP2 and MMP9 as well as inhibiting expression of E?cadherin and ??catenin. Twit Text FOAVip HIF 1 binding to AEG 1 promoter induced upregulated AEG 1 expression associated with metastasis in ovarian cancer ????Cancer Med http://www.kidney.de/pget.php?&tw=1&pmid=28401704 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28401704 #FOAvip English Wikipedia <ref>{{Cite pmid|28401704}}</ref> HIF?1? binding to AEG?1 promoter induced upregulated AEG?1 expression associated with metastasis in ovarian cancer #MMPMID28401704 Zhao T; Zhao C; Zhou Y; Zheng J; Gao S; Lu Y Cancer Med 2017[May]; 6 (5): 1072-81 PMID28401704show ga Ovarian cancer with the highest mortality rate among gynecological malignancies is one of common cancers among female cancer patients. As reported in recent years, AEG?1 was associated with the occurrence, development, and metastasis of ovarian cancer, but the mechanisms remain unclear. In the current study, invasion capabilities of ovarian cancer OVCAR3 cells were measured by viral infection and transwell assay. Western blot analysis was used to evaluate the expression levels of ??catenin, E?cadherin, MMP2, and MMP9. With qRT?PCR analysis, AEG?1 and HIF?1? gene expression were detected. We used luciferase reporter gene to measure AEG?1 promoter activity under normoxia/hypoxia in OVCAR3 cells. Our work demonstrated that AEG?1 significantly enhanced invasion capabilities of OVCAR3 cells and the expression levels of ??catenin, E?cadherin, MMP2, and MMP9 associated with invasion capabilities of OVCAR3 cells were upregulated. Furthermore, hypoxia enhanced invasion capabilities of OVCAR3 cells and induced AEG?1 high gene expression, which was reversed by AEG?1 knockdown lentivirus. HIF?1? expression upregulation was induced in OVCAR3 cells after hypoxia. HIF?1? knockdown lentivirus induced downregulated expression of AEG?1 and invasion capabilities of OVCAR3 cells were also inhibited. Wild?type AEG?1 promoter activity under hypoxic conditions was significantly higher than that AEG?1 mutation under normoxic conditions in the absence of hypoxia response. Our results suggested that HIF?1? binds to AEG?1 promoter to upregulate its expression, which was correlated with metastasis in ovarian cancer by inducing the expression of MMP2 and MMP9 as well as inhibiting expression of E?cadherin and ??catenin. äHIF?1? binding to AEG?1 promoter induced upregulated AEG?1 expression (epmc).pdf DeepDyve Pubget Overpricing