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10.1074/jbc.M117.782284 http://scihub22266oqcxt.onion/10.1074/jbc.M117.782284 C5427282!5427282!28325840     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2017 ; 292 (19): 8073-81 Nephropedia Template TP {{tp|p=28325840|t=2017. Assembly-induced folding regulates interleukin 12 biogenesis and secretion |pdf=|usr=}}{{28325840}} gab.com Text Assembly-induced folding regulates interleukin 12 biogenesis and secretion JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28325840 #FOAvip Members of the IL-12 family perform essential functions in immunoregulation by connecting innate and adaptive immunity and are emerging therapeutic targets. They are unique among other interleukins in forming heterodimers that arise from extensive subunit sharing within the family, leading to the production of at least four functionally distinct heterodimers from only five subunits. This raises important questions about how the assembly of IL-12 family members is regulated and controlled in the cell. Here, using cell-biological approaches, we have dissected basic principles that underlie the biogenesis of the founding member of the family, IL-12. Within the native IL-12 heterodimer, composed of IL-12? and IL-12?, IL-12? possesses three intramolecular and one intermolecular disulfide bridges. We show that, in isolation, IL-12? fails to form its native structure but, instead, misfolds, forming incorrect disulfide bonds. Co-expression of its ? subunit inhibits misfolding and thus allows secretion of biologically active heterodimeric IL-12. On the basis of these findings, we identified the disulfide bonds in IL-12? that are critical for assembly-induced secretion and biological activity of IL-12 versus misfolding and degradation of IL-12?. Surprisingly, two of the three disulfide bridges in IL-12? are dispensable for IL-12 secretion, stability, and biological activity. Extending our findings, we show that misfolding also occurs for IL-23?, another IL-12 family protein. Our results indicate that assembly-induced folding is key in IL-12 family biogenesis and secretion. The identification of essential disulfide bonds that underlie this process lays the basis for a simplified yet functional IL-12 cytokine. Twit Text FOAVip Assembly-induced folding regulates interleukin 12 biogenesis and secretion ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=28325840 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28325840 #FOAvip English Wikipedia <ref>{{Cite pmid|28325840}}</ref> Assembly-induced folding regulates interleukin 12 biogenesis and secretion #MMPMID28325840 Reitberger S; Haimerl P; Aschenbrenner I; Esser-von Bieren J; Feige MJ J Biol Chem 2017[May]; 292 (19): 8073-81 PMID28325840show ga Members of the IL-12 family perform essential functions in immunoregulation by connecting innate and adaptive immunity and are emerging therapeutic targets. They are unique among other interleukins in forming heterodimers that arise from extensive subunit sharing within the family, leading to the production of at least four functionally distinct heterodimers from only five subunits. This raises important questions about how the assembly of IL-12 family members is regulated and controlled in the cell. Here, using cell-biological approaches, we have dissected basic principles that underlie the biogenesis of the founding member of the family, IL-12. Within the native IL-12 heterodimer, composed of IL-12? and IL-12?, IL-12? possesses three intramolecular and one intermolecular disulfide bridges. We show that, in isolation, IL-12? fails to form its native structure but, instead, misfolds, forming incorrect disulfide bonds. Co-expression of its ? subunit inhibits misfolding and thus allows secretion of biologically active heterodimeric IL-12. On the basis of these findings, we identified the disulfide bonds in IL-12? that are critical for assembly-induced secretion and biological activity of IL-12 versus misfolding and degradation of IL-12?. Surprisingly, two of the three disulfide bridges in IL-12? are dispensable for IL-12 secretion, stability, and biological activity. Extending our findings, we show that misfolding also occurs for IL-23?, another IL-12 family protein. Our results indicate that assembly-induced folding is key in IL-12 family biogenesis and secretion. The identification of essential disulfide bonds that underlie this process lays the basis for a simplified yet functional IL-12 cytokine. äAssembly-induced folding regulates interleukin 12 biogenesis and secre(epmc).pdf DeepDyve Pubget Overpricing