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10.1016/j.stemcr.2017.04.001 http://scihub22266oqcxt.onion/10.1016/j.stemcr.2017.04.001 C5425728!5425728!28457889     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Reports 2017 ; 8 (5): 1312-28 Nephropedia Template TP {{tp|p=28457889|t=2017. 2i Maintains a Naive Ground State in ESCs through Two Distinct Epigenetic Mechanisms |pdf=|usr=}}{{28457889}} gab.com Text 2i Maintains a Naive Ground State in ESCs through Two Distinct Epigenetic Mechanisms JO: Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=28457889 #FOAvip Mouse embryonic stem cells (ESCs) are maintained in serum with leukemia inhibitory factor (LIF) to maintain self-renewal and pluripotency. Recently, a 2i culture method was reported using a combination of MEK inhibition (MEKi) and GSK3 inhibition (GSK3i) with LIF to maintain ESCs in a naive ground state. How 2i maintains a ground state of ESCs remains elusive. Here we show that MEKi and GSK3i maintain the ESC ground state by downregulating global DNA methylation through two distinct mechanisms. MEK1 phosphorylates JMJD2C for ubiquitin-mediated protein degradation. Therefore, MEKi increased JMJD2C protein levels but decreased DNMT3 expression. JMJD2C promotes TET1 activity to increase 5-hydroxymethylcytosine (5hmC) levels. GSK3i suppressed DNMT3 expression, thereby decreasing DNA methylation without affecting 5hmC levels. Furthermore, 2i increased PRDM14 expression to inhibit DNMT3A/B protein expression by promoting G9a-mediated DNMT3A/B protein degradation. Collectively, 2i allows ESCs to maintain a naive ground state through JMJD2C-dependent TET1 activation and PRDM14/G9a-mediated DNMT3A/B protein degradation. Twit Text FOAVip 2i Maintains a Naive Ground State in ESCs through Two Distinct Epigenetic Mechanisms ????Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=28457889 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28457889 #FOAvip English Wikipedia <ref>{{Cite pmid|28457889}}</ref> 2i Maintains a Naive Ground State in ESCs through Two Distinct Epigenetic Mechanisms #MMPMID28457889 Sim YJ; Kim MS; Nayfeh A; Yun YJ; Kim SJ; Park KT; Kim CH; Kim KS Stem Cell Reports 2017[May]; 8 (5): 1312-28 PMID28457889show ga Mouse embryonic stem cells (ESCs) are maintained in serum with leukemia inhibitory factor (LIF) to maintain self-renewal and pluripotency. Recently, a 2i culture method was reported using a combination of MEK inhibition (MEKi) and GSK3 inhibition (GSK3i) with LIF to maintain ESCs in a naive ground state. How 2i maintains a ground state of ESCs remains elusive. Here we show that MEKi and GSK3i maintain the ESC ground state by downregulating global DNA methylation through two distinct mechanisms. MEK1 phosphorylates JMJD2C for ubiquitin-mediated protein degradation. Therefore, MEKi increased JMJD2C protein levels but decreased DNMT3 expression. JMJD2C promotes TET1 activity to increase 5-hydroxymethylcytosine (5hmC) levels. GSK3i suppressed DNMT3 expression, thereby decreasing DNA methylation without affecting 5hmC levels. Furthermore, 2i increased PRDM14 expression to inhibit DNMT3A/B protein expression by promoting G9a-mediated DNMT3A/B protein degradation. Collectively, 2i allows ESCs to maintain a naive ground state through JMJD2C-dependent TET1 activation and PRDM14/G9a-mediated DNMT3A/B protein degradation. ä2i Maintains a Naive Ground State in ESCs through Two Distinct Epigene(epmc).pdf DeepDyve Pubget Overpricing