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10.1038/nmeth.4121 http://scihub22266oqcxt.onion/10.1038/nmeth.4121 C5423094!5423094!28024160     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2017 ; 14 (2): 201-7 Nephropedia Template TP {{tp|p=28024160|t=2017. RESA identifies mRNA regulatory sequences with high resolution |pdf=|usr=}}{{28024160}} gab.com Text RESA identifies mRNA regulatory sequences with high resolution JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip Gene expression is regulated extensively at the level of mRNA stability, localization, and translation. However, decoding functional RNA regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA Element Selection Assay (RESA), a method that selects RNA elements based on their activity in vivo and uses high-throughput sequencing to provide quantitative measurement of their regulatory function with near nucleotide resolution. We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. RESA provides a sensitive and quantitative measure of microRNA activity in vivo and also identifies novel regulatory sequences. To uncover specific sequence requirements within regulatory elements, we developed a bisulfite-mediated nucleotide conversion strategy for large-scale mutational analysis (RESA-bisulfite). Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP). The RESA platform can be broadly applicable to uncover the regulatory features shaping gene expression and cellular function. Twit Text FOAVip RESA identifies mRNA regulatory sequences with high resolution ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28024160 #FOAvip English Wikipedia <ref>{{Cite pmid|28024160}}</ref> RESA identifies mRNA regulatory sequences with high resolution #MMPMID28024160 Yartseva V; Takacs CM; Vejnar CE; Lee MT; Giraldez AJ Nat Methods 2017[Feb]; 14 (2): 201-7 PMID28024160show ga Gene expression is regulated extensively at the level of mRNA stability, localization, and translation. However, decoding functional RNA regulatory features remains a limitation to understanding post-transcriptional regulation in vivo. Here, we developed RNA Element Selection Assay (RESA), a method that selects RNA elements based on their activity in vivo and uses high-throughput sequencing to provide quantitative measurement of their regulatory function with near nucleotide resolution. We implemented RESA to identify sequence elements modulating mRNA stability during zebrafish embryogenesis. RESA provides a sensitive and quantitative measure of microRNA activity in vivo and also identifies novel regulatory sequences. To uncover specific sequence requirements within regulatory elements, we developed a bisulfite-mediated nucleotide conversion strategy for large-scale mutational analysis (RESA-bisulfite). Finally, we used the versatile RESA platform to map candidate protein-RNA interactions in vivo (RESA-CLIP). The RESA platform can be broadly applicable to uncover the regulatory features shaping gene expression and cellular function. äRESA identifies mRNA regulatory sequences with high resolution (epmc).pdf DeepDyve Pubget Overpricing