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10.1007/s12551-015-0179-1 http://scihub22266oqcxt.onion/10.1007/s12551-015-0179-1 C5418486!5418486!28510103     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biophys+Rev 2015 ; 7 (4): 379-90 Nephropedia Template TP {{tp|p=28510103|t=2015. Optically-controlled platforms for transfection and single- and sub-cellular surgery |pdf=|usr=}}{{28510103}} gab.com Text Optically-controlled platforms for transfection and single- and sub-cellular surgery JO: Biophys Rev http://www.kidney.de/pget.php?&tw=1&pmid=28510103 #FOAvip Improving the resolution of biological research to the single-cell or sub-cellular level is of critical importance in a wide variety of processes and disease conditions. Most obvious are those linked to aging and cancer, many of which are dependent upon stochastic processes where individual, unpredictable failures or mutations in individual cells can lead to serious downstream conditions across the whole organism. The traditional tools of biochemistry struggle to observe such processes: the vast majority are based upon ensemble approaches analysing the properties of bulk populations, which means that details of individual constituents is lost. What are required, then, are tools with the precision and resolution to probe and dissect cells at the single-micron scale: the scale of the individual organelles and structures that control their function. In this review, we highlight the use of highly-focused laser beams to create systems which provide precise control and specificity at the single-cell or even single-micron level. The intense focal points generated can directly interact with cells and cell membranes, which in conjunction with related modalities such as optical trapping provide a broad platform for the development of single-cell and sub-cellular surgery approaches. These highly tuneable tools have been demonstrated to deliver or remove material from cells of interest, and they can simultaneously excite fluorescent probes for imaging purposes or plasmonic structures for very local heating. We discuss both the history and recent applications of the field, highlighting the key findings and developments over the last 40 years of biophotonics research. Twit Text FOAVip Optically-controlled platforms for transfection and single- and sub-cellular surgery ????Biophys Rev http://www.kidney.de/pget.php?&tw=1&pmid=28510103 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28510103 #FOAvip English Wikipedia <ref>{{Cite pmid|28510103}}</ref> Optically-controlled platforms for transfection and single- and sub-cellular surgery #MMPMID28510103 Villangca M; Casey D; Glückstad J Biophys Rev 2015[Dec]; 7 (4): 379-90 PMID28510103show ga Improving the resolution of biological research to the single-cell or sub-cellular level is of critical importance in a wide variety of processes and disease conditions. Most obvious are those linked to aging and cancer, many of which are dependent upon stochastic processes where individual, unpredictable failures or mutations in individual cells can lead to serious downstream conditions across the whole organism. The traditional tools of biochemistry struggle to observe such processes: the vast majority are based upon ensemble approaches analysing the properties of bulk populations, which means that details of individual constituents is lost. What are required, then, are tools with the precision and resolution to probe and dissect cells at the single-micron scale: the scale of the individual organelles and structures that control their function. In this review, we highlight the use of highly-focused laser beams to create systems which provide precise control and specificity at the single-cell or even single-micron level. The intense focal points generated can directly interact with cells and cell membranes, which in conjunction with related modalities such as optical trapping provide a broad platform for the development of single-cell and sub-cellular surgery approaches. These highly tuneable tools have been demonstrated to deliver or remove material from cells of interest, and they can simultaneously excite fluorescent probes for imaging purposes or plasmonic structures for very local heating. We discuss both the history and recent applications of the field, highlighting the key findings and developments over the last 40 years of biophotonics research. äOptically-controlled platforms for transfection and single- and sub-ce(epmc).pdf DeepDyve Pubget Overpricing