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2010 ; 2
(3
): 121-135
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Multi-dimensional correlative imaging of subcellular events: combining the
strengths of light and electron microscopy
#MMPMID28510069
Su Y
; Nykanen M
; Jahn KA
; Whan R
; Cantrill L
; Soon LL
; Ratinac KR
; Braet F
Biophys Rev
2010[Aug]; 2
(3
): 121-135
PMID28510069
show ga
To genuinely understand how complex biological structures function, we must
integrate knowledge of their dynamic behavior and of their molecular machinery.
The combined use of light or laser microscopy and electron microscopy has become
increasingly important to our understanding of the structure and function of
cells and tissues at the molecular level. Such a combination of two or more
different microscopy techniques, preferably with different spatial- and
temporal-resolution limits, is often referred to as 'correlative microscopy'.
Correlative imaging allows researchers to gain additional novel
structure-function information, and such information provides a greater degree of
confidence about the structures of interest because observations from one method
can be compared to those from the other method(s). This is the strength of
correlative (or 'combined') microscopy, especially when it is combined with
combinatorial or non-combinatorial labeling approaches. In this topical review,
we provide a brief historical perspective of correlative microscopy and an
in-depth overview of correlative sample-preparation and imaging methods presently
available, including future perspectives on the trend towards integrative
microscopy and microanalysis.