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10.1007/s13238-017-0397-3 http://scihub22266oqcxt.onion/10.1007/s13238-017-0397-3 C5413600!5413600!28401346     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Protein+Cell 2017 ; 8 (5): 365-78 Nephropedia Template TP {{tp|p=28401346|t=2017. CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs |pdf=|usr=}}{{28401346}} gab.com Text CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs JO: Protein Cell http://www.kidney.de/pget.php?&tw=1&pmid=28401346 #FOAvip Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in a number of genes including SOD1 and FUS are associated with familial ALS. Here we report the generation of induced pluripotent stem cells (iPSCs) from fibroblasts of familial ALS patients bearing SOD1+/A272C and FUS+/G1566A mutations, respectively. We further generated gene corrected ALS iPSCs using CRISPR/Cas9 system. Genome-wide RNA sequencing (RNA-seq) analysis of motor neurons derived from SOD1+/A272C and corrected iPSCs revealed 899 aberrant transcripts. Our work may shed light on discovery of early biomarkers and pathways dysregulated in ALS, as well as provide a basis for novel therapeutic strategies to treat ALS.Electronic supplementary material: The online version of this article (doi:10.1007/s13238-017-0397-3) contains supplementary material, which is available to authorized users. Twit Text FOAVip CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs ????Protein Cell http://www.kidney.de/pget.php?&tw=1&pmid=28401346 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28401346 #FOAvip English Wikipedia <ref>{{Cite pmid|28401346}}</ref> CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs #MMPMID28401346 Wang L; Yi F; Fu L; Yang J; Wang S; Wang Z; Suzuki K; Sun L; Xu X; Yu Y; Qiao J; Belmonte JCI; Yang Z; Yuan Y; Qu J; Liu GH Protein Cell 2017[May]; 8 (5): 365-78 PMID28401346show ga Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in a number of genes including SOD1 and FUS are associated with familial ALS. Here we report the generation of induced pluripotent stem cells (iPSCs) from fibroblasts of familial ALS patients bearing SOD1+/A272C and FUS+/G1566A mutations, respectively. We further generated gene corrected ALS iPSCs using CRISPR/Cas9 system. Genome-wide RNA sequencing (RNA-seq) analysis of motor neurons derived from SOD1+/A272C and corrected iPSCs revealed 899 aberrant transcripts. Our work may shed light on discovery of early biomarkers and pathways dysregulated in ALS, as well as provide a basis for novel therapeutic strategies to treat ALS.Electronic supplementary material: The online version of this article (doi:10.1007/s13238-017-0397-3) contains supplementary material, which is available to authorized users. äCRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral s(epmc).pdf DeepDyve Pubget Overpricing