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10.1038/ncomms13889 http://scihub22266oqcxt.onion/10.1038/ncomms13889 C5412012!5412012!28004827     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2016 ; 7 (ä): ä Nephropedia Template TP {{tp|p=28004827|t=2016. HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels |pdf=|usr=}}{{28004827}} gab.com Text HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28004827 #FOAvip A fundamental question is how autophagosome formation is regulated. Here we show that the PX domain protein HS1BP3 is a negative regulator of autophagosome formation. HS1BP3 depletion increased the formation of LC3-positive autophagosomes and degradation of cargo both in human cell culture and in zebrafish. HS1BP3 is localized to ATG16L1- and ATG9-positive autophagosome precursors and we show that HS1BP3 binds phosphatidic acid (PA) through its PX domain. Furthermore, we find the total PA content of cells to be significantly upregulated in the absence of HS1BP3, as a result of increased activity of the PA-producing enzyme phospholipase D (PLD) and increased localization of PLD1 to ATG16L1-positive membranes. We propose that HS1BP3 regulates autophagy by modulating the PA content of the ATG16L1-positive autophagosome precursor membranes through PLD1 activity and localization. Our findings provide key insights into how autophagosome formation is regulated by a novel negative-feedback mechanism on membrane lipids. Twit Text FOAVip HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=28004827 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28004827 #FOAvip English Wikipedia <ref>{{Cite pmid|28004827}}</ref> HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels #MMPMID28004827 Holland P; Knævelsrud H; Søreng K; Mathai BJ; Lystad AH; Pankiv S; Bjørndal GT; Schultz SW; Lobert VH; Chan RB; Zhou B; Liestøl K; Carlsson SR; Melia TJ; Di Paolo G; Simonsen A Nat Commun 2016[]; 7 (ä): ä PMID28004827show ga A fundamental question is how autophagosome formation is regulated. Here we show that the PX domain protein HS1BP3 is a negative regulator of autophagosome formation. HS1BP3 depletion increased the formation of LC3-positive autophagosomes and degradation of cargo both in human cell culture and in zebrafish. HS1BP3 is localized to ATG16L1- and ATG9-positive autophagosome precursors and we show that HS1BP3 binds phosphatidic acid (PA) through its PX domain. Furthermore, we find the total PA content of cells to be significantly upregulated in the absence of HS1BP3, as a result of increased activity of the PA-producing enzyme phospholipase D (PLD) and increased localization of PLD1 to ATG16L1-positive membranes. We propose that HS1BP3 regulates autophagy by modulating the PA content of the ATG16L1-positive autophagosome precursor membranes through PLD1 activity and localization. Our findings provide key insights into how autophagosome formation is regulated by a novel negative-feedback mechanism on membrane lipids. äHS1BP3 negatively regulates autophagy by modulation of phosphatidic ac(epmc).pdf DeepDyve Pubget Overpricing