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10.1016/j.ymeth.2017.03.002 http://scihub22266oqcxt.onion/10.1016/j.ymeth.2017.03.002 C5411311!5411311!28263887     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods 2017 ; 118-119 (ä): 111-8 Nephropedia Template TP {{tp|p=28263887|t=2017. Mapping specificity landscapes of RNA-protein interactions by high throughput sequencing |pdf=|usr=}}{{28263887}} gab.com Text Mapping specificity landscapes of RNA-protein interactions by high throughput sequencing JO: Methods http://www.kidney.de/pget.php?&tw=1&pmid=28263887 #FOAvip To function in a biological setting, RNA binding proteins (RBPs) have to discriminate between alternative binding sites in RNAs. This discrimination can occur in the ground state of an RNA-protein binding reaction, in its transition state, or in both. The extent by which RBPs discriminate at these reaction states defines RBP specificity landscapes. Here, we describe the HiTS-Kin and HiTS-EQ techniques, which combine kinetic and equilibrium binding experiments with high throughput sequencing to quantitatively assess substrate discrimination for large numbers of substrate variants at ground and transition states of RNA-protein binding reactions. We discuss experimental design, practical considerations and data analysis and outline how a combination of HiTS-Kin and HiTS-EQ allows the mapping of RBP specificity landscapes. Twit Text FOAVip Mapping specificity landscapes of RNA-protein interactions by high throughput sequencing ????Methods http://www.kidney.de/pget.php?&tw=1&pmid=28263887 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28263887 #FOAvip English Wikipedia <ref>{{Cite pmid|28263887}}</ref> Mapping specificity landscapes of RNA-protein interactions by high throughput sequencing #MMPMID28263887 Jankowsky E; Harris ME Methods 2017[Apr]; 118-119 (ä): 111-8 PMID28263887show ga To function in a biological setting, RNA binding proteins (RBPs) have to discriminate between alternative binding sites in RNAs. This discrimination can occur in the ground state of an RNA-protein binding reaction, in its transition state, or in both. The extent by which RBPs discriminate at these reaction states defines RBP specificity landscapes. Here, we describe the HiTS-Kin and HiTS-EQ techniques, which combine kinetic and equilibrium binding experiments with high throughput sequencing to quantitatively assess substrate discrimination for large numbers of substrate variants at ground and transition states of RNA-protein binding reactions. We discuss experimental design, practical considerations and data analysis and outline how a combination of HiTS-Kin and HiTS-EQ allows the mapping of RBP specificity landscapes. äMapping specificity landscapes of RNA-protein interactions by high thr(epmc).pdf DeepDyve Pubget Overpricing