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10.1002/adfm.201500624

http://scihub22266oqcxt.onion/10.1002/adfm.201500624
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suck abstract from ncbi


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pmid28458628
      Adv+Funct+Mater 2015 ; 25 (21 ): 3183-3192
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  • Integrative Self-assembly of Graphene Quantum Dot and Biopolymer into a Versatile Biosensing Toolkit #MMPMID28458628
  • Lin Y ; Chapman R ; Stevens MM
  • Adv Funct Mater 2015[Jun]; 25 (21 ): 3183-3192 PMID28458628 show ga
  • Hybrid self-assembly has become a reliable approach to synthesize soft materials with multiple levels of structural complexity and synergistic functionality. In this work, photoluminescent graphene quantum dots (GQDs, 2-5 nm) are used for the first time as molecule-like building blocks to construct self-assembled hybrid materials for label-free biosensors. Ionic self-assembly of disc-shaped GQDs and charged biopolymers is found to generate a series of hierarchical structures that exhibit aggregation-induced fluorescence quenching of the GQDs and change the protein/polypeptide secondary structure. The integration of GQDs and biopolymers via self-assembly offers a flexible toolkit for the design of label-free biosensors in which the GQDs serve as a fluorescent probe and the biopolymers provide biological function. The versatility of this approach is demonstrated in the detection of glycosaminoglycans (GAGs), pH, and proteases using three strategies: 1) competitive binding of GAGs to biopolymers, 2) pH-responsive structural changes of polypeptides, and 3) enzymatic hydrolysis of the protein backbone, respectively. It is anticipated that the integrative self-assembly of biomolecules and GQDs will open up new avenues for the design of multifunctional biomaterials with combined optoelectronic properties and biological applications.
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