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2015 ; 25
(21
): 3183-3192
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gab.com Text
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English Wikipedia
Integrative Self-assembly of Graphene Quantum Dot and Biopolymer into a Versatile
Biosensing Toolkit
#MMPMID28458628
Lin Y
; Chapman R
; Stevens MM
Adv Funct Mater
2015[Jun]; 25
(21
): 3183-3192
PMID28458628
show ga
Hybrid self-assembly has become a reliable approach to synthesize soft materials
with multiple levels of structural complexity and synergistic functionality. In
this work, photoluminescent graphene quantum dots (GQDs, 2-5 nm) are used for the
first time as molecule-like building blocks to construct self-assembled hybrid
materials for label-free biosensors. Ionic self-assembly of disc-shaped GQDs and
charged biopolymers is found to generate a series of hierarchical structures that
exhibit aggregation-induced fluorescence quenching of the GQDs and change the
protein/polypeptide secondary structure. The integration of GQDs and biopolymers
via self-assembly offers a flexible toolkit for the design of label-free
biosensors in which the GQDs serve as a fluorescent probe and the biopolymers
provide biological function. The versatility of this approach is demonstrated in
the detection of glycosaminoglycans (GAGs), pH, and proteases using three
strategies: 1) competitive binding of GAGs to biopolymers, 2) pH-responsive
structural changes of polypeptides, and 3) enzymatic hydrolysis of the protein
backbone, respectively. It is anticipated that the integrative self-assembly of
biomolecules and GQDs will open up new avenues for the design of multifunctional
biomaterials with combined optoelectronic properties and biological applications.