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10.1016/j.molcel.2016.06.006 http://scihub22266oqcxt.onion/10.1016/j.molcel.2016.06.006 C5407370!5407370!27425413     free     free     free       Mol+Cell 2016 ; 63 (2): 329-36 Nephropedia Template TP {{tp|p=27425413|t=2016. Creating single-copy genetic circuits |pdf=|usr=}}{{27425413}} gab.com Text Creating single-copy genetic circuits JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27425413 #FOAvip Synthetic biology is increasingly used to develop sophisticated living devices for basic and applied research. Many of these genetic devices are engineered using multi-copy plasmids, but as the field progresses from proof-of-principle demonstrations to practical applications, it is important to develop single-copy synthetic modules that minimize consumption of cellular resources and can be stably maintained as genomic integrants. Here we use empirical design, mathematical modeling and iterative construction and testing to build single-copy, bistable toggle switches with improved performance and reduced metabolic load that can be stably integrated into the host genome. Deterministic and stochastic models led us to focus on basal transcription to optimize circuit performance and helped to explain the resulting circuit robustness across a large range of component expression levels. The design parameters developed here provide important guidance for future efforts to convert functional multi-copy gene circuits into optimized single-copy circuits for practical, real-world use. Twit Text FOAVip Creating single-copy genetic circuits ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=27425413 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27425413 #FOAvip English Wikipedia <ref>{{Cite pmid|27425413}}</ref> Creating single-copy genetic circuits #MMPMID27425413 Lee JW; Gyorgy A; Cameron DE; Pyenson N; Choi KR; Way JC; Silver PA; Del Vecchio D; Collins JJ Mol Cell 2016[Jul]; 63 (2): 329-36 PMID27425413show ga Synthetic biology is increasingly used to develop sophisticated living devices for basic and applied research. Many of these genetic devices are engineered using multi-copy plasmids, but as the field progresses from proof-of-principle demonstrations to practical applications, it is important to develop single-copy synthetic modules that minimize consumption of cellular resources and can be stably maintained as genomic integrants. Here we use empirical design, mathematical modeling and iterative construction and testing to build single-copy, bistable toggle switches with improved performance and reduced metabolic load that can be stably integrated into the host genome. Deterministic and stochastic models led us to focus on basal transcription to optimize circuit performance and helped to explain the resulting circuit robustness across a large range of component expression levels. The design parameters developed here provide important guidance for future efforts to convert functional multi-copy gene circuits into optimized single-copy circuits for practical, real-world use. äCreating single-copy genetic circuits (epmc).pdf DeepDyve Pubget Overpricing