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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 Am+J+Physiol+Renal+Physiol
2017 ; 312
(4
): F778-F790
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English Wikipedia
Zebrafish mesonephric renin cells are functionally conserved and comprise two
distinct morphological populations
#MMPMID28179256
Rider SA
; Christian HC
; Mullins LJ
; Howarth AR
; MacRae CA
; Mullins JJ
Am J Physiol Renal Physiol
2017[Apr]; 312
(4
): F778-F790
PMID28179256
show ga
Zebrafish provide an excellent model in which to assess the role of the
renin-angiotensin system in renal development, injury, and repair. In contrast to
mammals, zebrafish kidney organogenesis terminates with the mesonephros. Despite
this, the basic functional structure of the nephron is conserved across
vertebrates. The relevance of teleosts for studies relating to the regulation of
the renin-angiotensin system was established by assessing the phenotype and
functional regulation of renin-expressing cells in zebrafish. Transgenic
fluorescent reporters for renin (ren), smooth muscle actin (acta2), and
platelet-derived growth factor receptor-beta (pdgfrb) were studied to determine
the phenotype and secretory ultrastructure of perivascular renin-expressing
cells. Whole kidney ren transcription responded to altered salinity,
pharmacological renin-angiotensin system inhibition, and renal injury.
Mesonephric ren-expressing cells occupied niches at the preglomerular arteries
and afferent arterioles, forming intermittent epithelioid-like multicellular
clusters exhibiting a granular secretory ultrastructure. In contrast, renin cells
of the efferent arterioles were thin bodied and lacked secretory granules. Renin
cells expressed the perivascular cell markers acta2 and pdgfrb Transcriptional
responses of ren to physiological challenge support the presence of a functional
renin-angiotensin system and are consistent with the production of active renin.
The reparative capability of the zebrafish kidney was harnessed to demonstrate
that ren transcription is a marker for renal injury and repair. Our studies
demonstrate substantive conservation of renin regulation across vertebrates, and
ultrastructural studies of renin cells reveal at least two distinct morphologies
of mesonephric perivascular ren-expressing cells.
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