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10.1111/cas.13179 http://scihub22266oqcxt.onion/10.1111/cas.13179 C5406534!5406534 !28403548     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\28403548 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cancer+Sci 2017 ; 108 (4 ): 713-718 Nephropedia Template TP {{tp|p=28403548 |t=2017. Protein-arginine deiminase 2 suppresses proliferation of colon cancer cells through protein citrullination |pdf=|usr=}}{{28403548 }} gab.com Text Protein-arginine deiminase 2 suppresses proliferation of colon cancer cells through protein citrullination JO: Cancer Sci http://www.kidney.de/pget.php?&tw=1&pmid=28403548 #FOAvip Expression of the gene for protein-arginine deiminase 2 (PADI2) has been shown to be downregulated in colon cancer, with such downregulation being indicative of a poor prognosis in individuals with this disease. We have now examined the expression of PADI2 in matched colon cancer and normal colon tissue specimens as well as in colon cancer cell lines. We found that isoform 1 of PADI2 is the predominant isoform in colon tissue and is downregulated during colon carcinogenesis. Immunohistochemical analysis showed that PADI2 is expressed in normal colonic epithelial cells. Overexpression of PADI2 isoform 1 suppressed the proliferation of colon cancer cells in vitro in association with increased protein citrullination. Expression of a catalytically inactive mutant (C647A) of PADI2 or of PADI2 isoform 2 did not induce such effects, indicating that the protein citrullination activity of PADI2 is required for inhibition of cell growth. The growth defect induced by PADI2 was not attributable to increased apoptosis but rather was accompanied by arrest of cell cycle progression in G(1) phase. Finally, we detected citrullinated proteins in normal colon tissue by immunoblot analysis. Our data thus suggest that PADI2 suppresses the proliferation of colonic epithelial cells through catalysis of protein citrullination, and that downregulation of PADI2 expression might therefore contribute to colon carcinogenesis. Twit Text FOAVip Protein-arginine deiminase 2 suppresses proliferation of colon cancer cells through protein citrullination ????Cancer Sci http://www.kidney.de/pget.php?&tw=1&pmid=28403548 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28403548 #FOAvip English Wikipedia <ref>{{Cite pmid|28403548 }}</ref> Protein-arginine deiminase 2 suppresses proliferation of colon cancer cells through protein citrullination #MMPMID28403548 Funayama R ; Taniguchi H ; Mizuma M ; Fujishima F ; Kobayashi M ; Ohnuma S ; Unno M ; Nakayama K Cancer Sci 2017[Apr]; 108 (4 ): 713-718 PMID28403548 show ga Expression of the gene for protein-arginine deiminase 2 (PADI2) has been shown to be downregulated in colon cancer, with such downregulation being indicative of a poor prognosis in individuals with this disease. We have now examined the expression of PADI2 in matched colon cancer and normal colon tissue specimens as well as in colon cancer cell lines. We found that isoform 1 of PADI2 is the predominant isoform in colon tissue and is downregulated during colon carcinogenesis. Immunohistochemical analysis showed that PADI2 is expressed in normal colonic epithelial cells. Overexpression of PADI2 isoform 1 suppressed the proliferation of colon cancer cells in vitro in association with increased protein citrullination. Expression of a catalytically inactive mutant (C647A) of PADI2 or of PADI2 isoform 2 did not induce such effects, indicating that the protein citrullination activity of PADI2 is required for inhibition of cell growth. The growth defect induced by PADI2 was not attributable to increased apoptosis but rather was accompanied by arrest of cell cycle progression in G(1) phase. Finally, we detected citrullinated proteins in normal colon tissue by immunoblot analysis. Our data thus suggest that PADI2 suppresses the proliferation of colonic epithelial cells through catalysis of protein citrullination, and that downregulation of PADI2 expression might therefore contribute to colon carcinogenesis. |*Gene Expression Regulation, Neoplastic [MESH] |Adult [MESH] |Aged [MESH] |Cell Line, Tumor [MESH] |Cell Proliferation/*genetics [MESH] |Citrulline/metabolism [MESH] |Colonic Neoplasms/*genetics/metabolism/pathology [MESH] |Down-Regulation [MESH] |Female [MESH] |G1 Phase Cell Cycle Checkpoints/genetics [MESH] |HCT116 Cells [MESH] |Humans [MESH] |Hydrolases/*genetics/metabolism [MESH] |Immunoblotting [MESH] |Immunohistochemistry [MESH] |Isoenzymes/genetics/metabolism [MESH] |Male [MESH] |Middle Aged [MESH] |Mutation [MESH] |Protein-Arginine Deiminase Type 2 [MESH] |Protein-Arginine Deiminases [MESH] |RNA Interference [MESH]Protein-arginine deiminase 2 suppresses proliferation of colon cancer (epmc).pdf DeepDyve Pubget Overpricing