Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1111/apha.12528 http://scihub22266oqcxt.onion/10.1111/apha.12528 C5406130!5406130!25980432     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Acta+Physiol+(Oxf) 2015 ; 214 (3): 319-28 Nephropedia Template TP {{tp|p=25980432|t=2015. TRPV4 mediates flow-induced increases in intracellular Ca in medullary thick ascending limbs |pdf=|usr=}}{{25980432}} gab.com Text TRPV4 mediates flow-induced increases in intracellular Ca in medullary thick ascending limbs JO: Acta Physiol (Oxf) http://www.kidney.de/pget.php?&tw=1&pmid=25980432 #FOAvip Medullary thick ascending limbs (mTAL) regulate Na balance and therefore blood pressure. We previously showed that cell swelling and luminal flow activates the mechano-sensitive channel TRPV4 in mTAL.Aim: We hypothesized that TRPV4 mediates flow-induced increases in intracellular Ca (Cai) in rat mTALs. Methods: We performed ratiometric measurements of Cai in perfused mTALs. Results: Increasing luminal flow from 0 to 20 nL min-1 caused Cai to peak 231±29 nmol L-1 above basal concentrations (n=18). The general TRPV inhibitor ruthenium red at 15 and 50 umol L-1 reduced peak Cai by 41±9 (p<0.01;n=5) and 77±10 % (p<0.02;n=6). The selective TRPV4 inhibitor RN1734 at 10 and 50 umol L-1 reduced peak Cai by 46±11 (p<0.01;n=7) and 76±5% (p<0.02;n=5) respectively. To specifically target TRPV4, mTALs were transduced with adenoviruses expressing TRPV4 small hairpin (sh) RNA. In non-transduced control mTALs, luminal flow generated a peak increase in Cai of 111±21 nmol L-1 (n=8). In TRPV4shRNA-transduced mTALs, the Cai peak was reduced to 56±8 nmol L-1 (p<0.03,n=9). Removing extracellular Ca completely abolished flow-induced increases in Cai. Increasing luminal flow in the presence of hexokinase 20 (U/ml) to scavenge extracellular ATP did not modify significantly the increases in Cai induced by luminal flow. Finally, we studied the effect of the TRPV4 selective agonist GSK1016790A on Cai. In the absence of luminal flow, GSK1016790A (10 nmol L-1) increased Cai from 60±11 nmol L-1 to 262±71 nmol L-1 (p<0.05;n=7). Conclusion: We conclude that flow-induced increases in Cai are mediated primarily by TRPV4 in the rat mTAL. Twit Text FOAVip TRPV4 mediates flow-induced increases in intracellular Ca in medullary thick ascending limbs ????Acta Physiol (Oxf) http://www.kidney.de/pget.php?&tw=1&pmid=25980432 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25980432 #FOAvip English Wikipedia <ref>{{Cite pmid|25980432}}</ref> TRPV4 mediates flow-induced increases in intracellular Ca in medullary thick ascending limbs #MMPMID25980432 Cabral PD; Capurro C; Garvin JL Acta Physiol (Oxf) 2015[Jul]; 214 (3): 319-28 PMID25980432show ga Medullary thick ascending limbs (mTAL) regulate Na balance and therefore blood pressure. We previously showed that cell swelling and luminal flow activates the mechano-sensitive channel TRPV4 in mTAL.Aim: We hypothesized that TRPV4 mediates flow-induced increases in intracellular Ca (Cai) in rat mTALs. Methods: We performed ratiometric measurements of Cai in perfused mTALs. Results: Increasing luminal flow from 0 to 20 nL min-1 caused Cai to peak 231±29 nmol L-1 above basal concentrations (n=18). The general TRPV inhibitor ruthenium red at 15 and 50 umol L-1 reduced peak Cai by 41±9 (p<0.01;n=5) and 77±10 % (p<0.02;n=6). The selective TRPV4 inhibitor RN1734 at 10 and 50 umol L-1 reduced peak Cai by 46±11 (p<0.01;n=7) and 76±5% (p<0.02;n=5) respectively. To specifically target TRPV4, mTALs were transduced with adenoviruses expressing TRPV4 small hairpin (sh) RNA. In non-transduced control mTALs, luminal flow generated a peak increase in Cai of 111±21 nmol L-1 (n=8). In TRPV4shRNA-transduced mTALs, the Cai peak was reduced to 56±8 nmol L-1 (p<0.03,n=9). Removing extracellular Ca completely abolished flow-induced increases in Cai. Increasing luminal flow in the presence of hexokinase 20 (U/ml) to scavenge extracellular ATP did not modify significantly the increases in Cai induced by luminal flow. Finally, we studied the effect of the TRPV4 selective agonist GSK1016790A on Cai. In the absence of luminal flow, GSK1016790A (10 nmol L-1) increased Cai from 60±11 nmol L-1 to 262±71 nmol L-1 (p<0.05;n=7). Conclusion: We conclude that flow-induced increases in Cai are mediated primarily by TRPV4 in the rat mTAL. äTRPV4 mediates flow-induced increases in intracellular Ca in medullary(epmc).pdf DeepDyve Pubget Overpricing