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10.1016/j.chembiol.2017.03.001 http://scihub22266oqcxt.onion/10.1016/j.chembiol.2017.03.001 C5404835!5404835!28366620     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Chem+Biol ä ; 24 (4): 525-531.e4 Nephropedia Template TP {{tp|p=28366620|t=ä. A Ratiometric Sensor for Imaging Insulin Secretion in Single ? Cells |pdf=|usr=}}{{28366620}} gab.com Text A Ratiometric Sensor for Imaging Insulin Secretion in Single Cells JO: Cell Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=28366620 #FOAvip Despite the urgent need for assays to visualize insulin secretion there is to date no reliable method available for measuring insulin release from single cells. To address this need, we developed a genetically encoded reporter termed RINS1 based on proinsulin superfolder GFP (sfGFP) and mCherry fusions for monitoring insulin secretion. RINS1 expression in MIN6 ? cells resulted in proper processing yielding single-labeled insulin species. Unexpectedly, glucose or drug stimulation of insulin secretion in ? cells led to the preferential release of the insulin-sfGFP construct, while the mCherry-fused C-peptide remained trapped in exocytic granules. This physical separation was used to monitor glucose-stimulated insulin secretion ratiometrically by total internal reflection fluorescence microscopy in single MIN6 and primary mouse ? cells. Further, RINS1 enabled parallel monitoring of pulsatile insulin release in tolbutamide-treated ? cells, demonstrating the potential of RINS1 for investigations of antidiabetic drug candidates at the single-cell level. Twit Text FOAVip A Ratiometric Sensor for Imaging Insulin Secretion in Single Cells ????Cell Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=28366620 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28366620 #FOAvip English Wikipedia <ref>{{Cite pmid|28366620}}</ref> A Ratiometric Sensor for Imaging Insulin Secretion in Single ? Cells #MMPMID28366620 Schifferer M; Yushchenko DA; Stein F; Bolbat A; Schultz C Cell Chem Biol ä[]; 24 (4): 525-531.e4 PMID28366620show ga Despite the urgent need for assays to visualize insulin secretion there is to date no reliable method available for measuring insulin release from single cells. To address this need, we developed a genetically encoded reporter termed RINS1 based on proinsulin superfolder GFP (sfGFP) and mCherry fusions for monitoring insulin secretion. RINS1 expression in MIN6 ? cells resulted in proper processing yielding single-labeled insulin species. Unexpectedly, glucose or drug stimulation of insulin secretion in ? cells led to the preferential release of the insulin-sfGFP construct, while the mCherry-fused C-peptide remained trapped in exocytic granules. This physical separation was used to monitor glucose-stimulated insulin secretion ratiometrically by total internal reflection fluorescence microscopy in single MIN6 and primary mouse ? cells. Further, RINS1 enabled parallel monitoring of pulsatile insulin release in tolbutamide-treated ? cells, demonstrating the potential of RINS1 for investigations of antidiabetic drug candidates at the single-cell level. äA Ratiometric Sensor for Imaging Insulin Secretion in Single ? Cells (epmc).pdf DeepDyve Pubget Overpricing