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Novel role of granulocyte-macrophage colony-stimulating factor: antitumor effects
through inhibition of epithelial-to-mesenchymal transition in esophageal cancer
#MMPMID28461757
Zhang J
; Liu Q
; Qiao L
; Hu P
; Deng G
; Liang N
; Xie J
; Luo H
; Zhang J
Onco Targets Ther
2017[]; 10
(?): 2227-2237
PMID28461757
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PURPOSE: Recent studies demonstrate the possible antitumor effects of
granulocyte-macrophage colony-stimulating factor (GM-CSF); however, the exact
mechanism is still unclear. The aim of our study was to analyze the effects of
GM-CSF on multiple biological functions of human esophageal cancer (EC) cell
lines and to explore the potential mechanism of its antitumor effects. MATERIALS
AND METHODS: Eca109/9706 human EC cells were examined. Cell proliferation,
apoptosis, and migration were analyzed using cell proliferation assay, flow
cytometry, and transwell assay, respectively. The expression of signaling
molecules were examined by reverse transcription polymerase chain reaction and
Western blot. RESULTS: Our results provide experimental evidence that GM-CSF
inhibits growth and migration, as well as induction of apoptosis in EC cells. In
addition, EC cells stimulated with GM-CSF were more likely to have suppressed
epithelial-to-mesenchymal transition (EMT), accompanied by increased E-cadherin
and decreased vimentin expression. CONCLUSION: Our data demonstrate that GM-CSF
inhibits cancer cell proliferation and migration, as well as induction of
apoptosis. Moreover, our findings indicate that GM-CSF may regulate EMT through
JAK2-PRMT5 signaling, and thereby exhibit its antitumor effects on EC cells.
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