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10.1371/journal.pone.0176221 http://scihub22266oqcxt.onion/10.1371/journal.pone.0176221 C5404769!5404769!28441427     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2017 ; 12 (4): ä Nephropedia Template TP {{tp|p=28441427|t=2017. Programmable type III-A CRISPR-Cas DNA targeting modules |pdf=|usr=}}{{28441427}} gab.com Text Programmable type III-A CRISPR-Cas DNA targeting modules JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28441427 #FOAvip The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of Csm systems from 3 bacterial species and heterologously expressed the functional modules in E. coli. The modules include a Cas6 protein and a CRISPR locus for crRNA production, and Csm effector complex proteins. The expressed modules from L. lactis, S. epidermidis and S. thermophilus specifically eliminate invading plasmids recognized by the crRNAs of the systems. Characteristically, activation of plasmid targeting activity depends on transcription of the plasmid sequence recognized by the crRNA. Activity was not observed when transcription of the crRNA target sequence was blocked, or when the opposite strand or a non-target sequence was transcribed. Moreover, the Csm module can be programmed to recognize plasmids with novel target sequences by addition of appropriate crRNA coding sequences to the module. These systems provide a platform for investigation of Type III-A CRISPR-Cas systems in E. coli, and for introduction of programmable transcription-activated DNA targeting into novel organisms. Twit Text FOAVip Programmable type III-A CRISPR-Cas DNA targeting modules ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=28441427 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28441427 #FOAvip English Wikipedia <ref>{{Cite pmid|28441427}}</ref> Programmable type III-A CRISPR-Cas DNA targeting modules #MMPMID28441427 Ichikawa HT; Cooper JC; Lo L; Potter J; Terns RM; Terns MP PLoS One 2017[]; 12 (4): ä PMID28441427show ga The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of Csm systems from 3 bacterial species and heterologously expressed the functional modules in E. coli. The modules include a Cas6 protein and a CRISPR locus for crRNA production, and Csm effector complex proteins. The expressed modules from L. lactis, S. epidermidis and S. thermophilus specifically eliminate invading plasmids recognized by the crRNAs of the systems. Characteristically, activation of plasmid targeting activity depends on transcription of the plasmid sequence recognized by the crRNA. Activity was not observed when transcription of the crRNA target sequence was blocked, or when the opposite strand or a non-target sequence was transcribed. Moreover, the Csm module can be programmed to recognize plasmids with novel target sequences by addition of appropriate crRNA coding sequences to the module. These systems provide a platform for investigation of Type III-A CRISPR-Cas systems in E. coli, and for introduction of programmable transcription-activated DNA targeting into novel organisms. äProgrammable type III-A CRISPR-Cas DNA targeting modules (epmc).pdf DeepDyve Pubget Overpricing