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2017 ; 10
(3
): 570-585
Nephropedia Template TP
Microb Biotechnol
2017[May]; 10
(3
): 570-585
PMID28224723
show ga
Synthetic extreme environments like carwash effluent tanks and drains are
potential sources of biotechnologically important microorganisms and molecules
which have, however, remained unexplored. Using culture- and molecular-based
methods, a total of 17 bacterial isolates belonging to the genera Shewanella,
Proteus, Paenibacillus, Enterobacter and Citrobacter, Aeromonas, Pseudomonas and
Pantoea were identified. Hydrocarbon utilization and enzyme production screening
assays showed that Aeromonas sp. CAC11, Paenibacillus sp. CAC12 and Paenibacillus
sp. CAC13 and Citrobacter sp. PCW7 were able to degrade benzanthracene,
naphthalene and diesel oil, Paenibacillus sp. CAC12 and Paenibacillus sp. CAC13
could produce cellulase enzyme, while Proteus sp. BPS2, Pseudomonas sp. SAS8 and
Proteus sp. CAL3 could produce lipase. GC-MS analysis of bacterial secondary
metabolites resulted in identification of 107 different compounds produced by
Proteus sp. BPS2, Paenibacillus sp. CAC12, Pseudomonas sp. SAS8, Proteus sp. CAL3
and Paenibacillus sp. CAC13. Most of the compounds identified by both GC-MS and
LC-MS have previously been determined to have antibacterial, antifungal and/or
anticancer properties. Further, microbial metabolites which have previously been
known to be produced only by plants or microorganisms found in natural extreme
environments were also identified in this study. This research has revealed the
immense bioresource potential of microorganisms inhabiting synthetic extreme
environments.