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miR-150 is downregulated in osteosarcoma and suppresses cell proliferation,
migration and invasion by targeting ROCK1
#MMPMID28454380
Li CH
; Yu TB
; Qiu HW
; Zhao X
; Zhou CL
; Qi C
Oncol Lett
2017[Apr]; 13
(4
): 2191-2197
PMID28454380
show ga
Osteosarcoma (OS) is the most common form of bone malignancy in children and
adolescents. A class of molecules known as microRNAs (miRNAs) have been routinely
associated in the development and progression of OS. The present study was
centered on the less well-known miRNA, miRNA (miR)-150, and its role in OS was
investigated. The levels of miR-150 were examined in 40 tissue specimens from
patients with OS and adjacent normal tissues using reverse
transcription-quantitative polymerase chain reaction (RT-qPCR) analysis. In
addition the expression levels of miR-150 were examined in three OS cell lines
and a normal osteoblast cell line. Cell proliferation, migration and invasion
assays were performed to establish the correlation between miR-150 and
metastasis. The potential targets of miR-150 were theoretically predicted and one
high-scoring target, Rho-associated kinase 1 (ROCK1), was established to be a
direct target using RT-qPCR and western blot analyses and Pearson's correlation
analysis. The results indicated that miR-150 was downregulated in tissues from
patients with OS and cell lines. Secondly, it was shown that the overexpression
of miR-150 was inversely correlated with OS cell proliferation, migration and
invasion. It was also shown that miR-150 negatively regulated the gene expression
of ROCK1 in the OS cell lines. Finally, the interaction between miR-150 and ROCK1
was established and it was shown that miR-150 directly targeted ROCK1. In
conclusion, miR-150 was found to be a tumor suppressor, and the suppression of
miR-150 resulted in elevation in the levels of ROCK1. This interaction between
miR-150 and ROCK1 may be key in the progression of OS. Furthermore, miR-150 or
ROCK1 may be potential therapeutic targets for the treatment of OS.
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