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2017 ; 13
(4
): 2281-2289
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KLF7 overexpression in human oral squamous cell carcinoma promotes migration and
epithelial-mesenchymal transition
#MMPMID28454392
Ding X
; Wang X
; Gong Y
; Ruan H
; Sun Y
; Yu Y
Oncol Lett
2017[Apr]; 13
(4
): 2281-2289
PMID28454392
show ga
Krüppel-like factor 7 (KLF7) is a member of the KLF family of zinc finger
transcription factors, and was the first KLF cloned using complementary DNA and
polymerase chain reaction (PCR) techniques with human vascular endothelial cells
as a template. In addition, KLF7 is known as the ubiquitous Krüppel-like factor,
as it is widely expressed in numerous human tissues at low levels. In the present
study, the function of KLF7 in migration and epithelial-mesenchymal transition
(EMT), which are associated with tumor progression, was investigated in human
oral squamous cell carcinoma (OSCC) cells. Genes that were differentially
expressed in normal vs. OSCC tissue were identified in the Gene Expression
Omnibus database, which identified upregulation of KLF7 in OSCC. The expression
and subcellular location of KLF7 was then analyzed using immunohistochemistry.
KLF7 expression was measured in three OSCC cell lines, and the two cell lines
with the highest (HN13) and lowest (CAL27) KLF7 expression were selected for
further analysis. Subsequently, HN13 cells with reduced KLF7 expression (sh-HN13)
and CAL27 cells overexpressing KLF7 (OE-CAL27) were constructed. Transwell
migration and wound healing assays were then used to analyze the migration of the
cells. In addition, mRNA and protein expression levels of the EMT markers
E-cadherin, N-cadherin, vimentin and snail were detected using reverse
transcription-quantitative PCR and western blotting. KLF7 overexpression in OSCC
was validated using tissue immunohistochemistry, which identified moderate to
high cytoplasmic staining of KLF7 in OSCC cells. KLF7 knockdown and
overexpression altered the migration ability of sh-HN13 and OE-CAL27 cells, which
decreased and increased significantly respectively. Expression of E-cadherin,
N-cadherin, vimentin and snail was markedly altered in sh-HN13 and OE-CAL27
cells, indicating changes in EMT status. The results of the present study suggest
that KLF7 overexpression changes the migratory behavior of OSCC cells, and
induces EMT and lymph node metastasis through the expression of snail.
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