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10.1016/j.stem.2016.07.004 http://scihub22266oqcxt.onion/10.1016/j.stem.2016.07.004 C5402355!5402355 !27524439     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=27524439 &cmd=llinks): Failed to open stream: HTTP request failed! 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Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators |pdf=|usr=}}{{27524439 }} gab.com Text Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators JO: Cell Stem Cell http://www.kidney.de/pget.php?&tw=1&pmid=27524439 #FOAvip Physiological stem cell function is regulated by secreted factors produced by niche cells. In this study, we describe an unbiased approach based on the differential single-cell gene expression analysis of mesenchymal osteolineage cells close to, and further removed from, hematopoietic stem/progenitor cells (HSPCs) to identify candidate niche factors. Mesenchymal cells displayed distinct molecular profiles based on their relative location. We functionally examined, among the genes that were preferentially expressed in proximal cells, three secreted or cell-surface molecules not previously connected to HSPC biology-the secreted RNase angiogenin, the cytokine IL18, and the adhesion molecule Embigin-and discovered that all of these factors are HSPC quiescence regulators. Therefore, our proximity-based differential single-cell approach reveals molecular heterogeneity within niche cells and can be used to identify novel extrinsic stem/progenitor cell regulators. Similar approaches could also be applied to other stem cell/niche pairs to advance the understanding of microenvironmental regulation of stem cell function. Twit Text FOAVip Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators ????Cell Stem Cell http://www.kidney.de/pget.php?&tw=1&pmid=27524439 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=27524439 #FOAvip English Wikipedia <ref>{{Cite pmid|27524439 }}</ref> Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators #MMPMID27524439 Silberstein L ; Goncalves KA ; Kharchenko PV ; Turcotte R ; Kfoury Y ; Mercier F ; Baryawno N ; Severe N ; Bachand J ; Spencer JA ; Papazian A ; Lee D ; Chitteti BR ; Srour EF ; Hoggatt J ; Tate T ; Lo Celso C ; Ono N ; Nutt S ; Heino J ; Sipilä K ; Shioda T ; Osawa M ; Lin CP ; Hu GF ; Scadden DT Cell Stem Cell 2016[Oct]; 19 (4 ): 530-543 PMID27524439 show ga Physiological stem cell function is regulated by secreted factors produced by niche cells. In this study, we describe an unbiased approach based on the differential single-cell gene expression analysis of mesenchymal osteolineage cells close to, and further removed from, hematopoietic stem/progenitor cells (HSPCs) to identify candidate niche factors. Mesenchymal cells displayed distinct molecular profiles based on their relative location. We functionally examined, among the genes that were preferentially expressed in proximal cells, three secreted or cell-surface molecules not previously connected to HSPC biology-the secreted RNase angiogenin, the cytokine IL18, and the adhesion molecule Embigin-and discovered that all of these factors are HSPC quiescence regulators. Therefore, our proximity-based differential single-cell approach reveals molecular heterogeneity within niche cells and can be used to identify novel extrinsic stem/progenitor cell regulators. Similar approaches could also be applied to other stem cell/niche pairs to advance the understanding of microenvironmental regulation of stem cell function. |*Stem Cell Niche [MESH] |Animals [MESH] |Bone Marrow Cells/cytology [MESH] |Bone and Bones/cytology [MESH] |Cell Lineage/genetics [MESH] |Cell Self Renewal/genetics [MESH] |Cell Separation [MESH] |Gene Deletion [MESH] |Gene Expression Profiling [MESH] |Hematopoietic Stem Cells/*cytology/metabolism [MESH] |Interleukin-18/metabolism [MESH] |Membrane Glycoproteins/metabolism [MESH] |Ribonuclease, Pancreatic/metabolism [MESH] |Single-Cell Analysis/*methods [MESH] |Time Factors [MESH] |Transcription, Genetic [MESH]Proximity-Based Differential Single-Cell Analysis of the Niche to Iden(epmc).pdf DeepDyve Pubget Overpricing