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10.1242/jcs.193912 http://scihub22266oqcxt.onion/10.1242/jcs.193912 C5399779!5399779!28202688     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Cell+Sci 2017 ; 130 (7): 1299-306 Nephropedia Template TP {{tp|p=28202688|t=2017. Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex |pdf=|usr=}}{{28202688}} gab.com Text Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex JO: J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=28202688 #FOAvip The nuclear pore complex (NPC), composed of ?30 different nucleoporins (Nups), is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring scaffold perforates the nuclear envelope and features a unique molecular machinery that regulates nucleocytoplasmic transport. However, the precise copy number and the spatial location of each Nup in the native NPC remain obscure due to the inherent difficulty of counting and localizing proteins inside of the sub-micrometer supramolecular complex. Here, we combined super-resolution single-point edge-excitation subdiffraction (SPEED) microscopy and nanobody-specific labeling to reveal the spatial distribution of scaffold Nups within three separate layers in the native NPC with a precision of ?3?nm. Our data reveal both the radial and axial spatial distributions for Pom121, Nup37 and Nup35 and provide evidence for their copy numbers of 8, 32 and 16, respectively, per NPC. This approach can help pave the path for mapping the entirety of Nups in native NPCs and also other structural components of macromolecular complexes. Twit Text FOAVip Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex ????J Cell Sci http://www.kidney.de/pget.php?&tw=1&pmid=28202688 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=28202688 #FOAvip English Wikipedia <ref>{{Cite pmid|28202688}}</ref> Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex #MMPMID28202688 Ma J; Kelich JM; Junod SL; Yang W J Cell Sci 2017[Apr]; 130 (7): 1299-306 PMID28202688show ga The nuclear pore complex (NPC), composed of ?30 different nucleoporins (Nups), is one of the largest supramolecular structures in eukaryotic cells. Its octagonal ring scaffold perforates the nuclear envelope and features a unique molecular machinery that regulates nucleocytoplasmic transport. However, the precise copy number and the spatial location of each Nup in the native NPC remain obscure due to the inherent difficulty of counting and localizing proteins inside of the sub-micrometer supramolecular complex. Here, we combined super-resolution single-point edge-excitation subdiffraction (SPEED) microscopy and nanobody-specific labeling to reveal the spatial distribution of scaffold Nups within three separate layers in the native NPC with a precision of ?3?nm. Our data reveal both the radial and axial spatial distributions for Pom121, Nup37 and Nup35 and provide evidence for their copy numbers of 8, 32 and 16, respectively, per NPC. This approach can help pave the path for mapping the entirety of Nups in native NPCs and also other structural components of macromolecular complexes. äSuper-resolution mapping of scaffold nucleoporins in the nuclear pore (epmc).pdf DeepDyve Pubget Overpricing